Abstract: 【Objective】To explore the effects of replacing the traditional soybean leaf with Pueraria lobata leaf on growth performance and intestinal microflora diversity of Clanis bilineata tingtauica larvae.【Methods】In this experiment,0-day-old larvae with the similar size and health level were fed with Pueraria lobata leaf（experimental group）and soybean leaf（control group）respectively. Each group was set up with 3 replicates and each replicate comprised 200 larvae. The differences in growth performance,survival rate,digested feed conversion efficiency,feed to gain ratio,nutritional composition and intestinal microflora diversity of larvae were compared and analyzed.【Results】Compared with soybean leaf,Pueraria lobata leaf fed larvae had higher survival rate（57.54%）,higher digested feed conversion efficiency（0.24 g/g）and lower feed to gain ratio（1.90 g/g）. Larvae in the group fed with Pueraria lobata leaf had better growth performance. The average body length（72.27 mm）,average body width（11.99 mm）and average body weight（7.34 g）of the 25-day-old Clanis bilineata tingtauica were all higher than those in the group fed with soybean leaf. Larvae（dry weight）fed with Pueraria lobata leaf had higher content of crude protein（64.12%）,crude fat（18.15%）and higher proportion of essential amino acids in total amino acids（37.72%）compared with those fed with soybean leaf. According to the 16S rRNA sequencing results,Clanis bilineata tingtauica larvae fed with Pueraria lobata leaf had more diverse intestinal microflora than larvae fed with soybean leaf,which was primarily made up of Firmicutes and several beneficial bacterial genus.【Conclusion】Considering the larval quality and economic benefits,the substitution of Pueraria lobata leaf for soybean leaf improved the growth performance and diversity of intestinal microflora of Clanis bilineata tingtauica larvae.

Key words: Clanis bilineata tingtauica larvae, Pueraria lobata leaf, Soybean leaf, Growth performance, Diversity of intestinal microflora