畜牧与饲料科学 ›› 2009, Vol. 30 ›› Issue (3): 51-51.doi: 10.12160/j.issn.1672-5190.2009.03.023

• 饲料科学与动物营养 • 上一篇    下一篇

SPE-HPLC测定饲料中的苯甲酸

孙淑红 姜玲玲 赵艳丽   

  1. 鞍山市产品质量监督检验所,辽宁鞍山114001
  • 出版日期:2009-03-20 发布日期:2009-03-20
  • 通讯作者: 孙淑红
  • 作者简介:孙淑红(1975-),女,工程师,主要研究方向为食品与饲料检验。

Determination on Benzoic Acid in Feed by SPE-HPLC

SUN Shu-hong, JIANG Ling-ling, ZHAO Yan-li (Anshan Product Quality Supervision and Testing Institute, Anshan 114001 ,China)   

  • Online:2009-03-20 Published:2009-03-20

摘要: 建立了饲料中苯甲酸的反相高效液相色谱(RP-HPLC)测定方法。经甲醇提取饲料中的苯甲酸,以C18固相萃取小柱进行纯化后,用反相高效液相色谱法检测。苯甲酸在50~250g/L范围内线性关系良好,饲料中苯甲酸的最低检测量为0.006g/kg。

Abstract: The benzoic acid in feed was determined by reversed-phase high performance liquid chromatography (RP-HPLC) and the determination method was established. Benzoic acid in feed was extracted by methanol, purified by C18 solid phase extraction column and detected by RP-HPLC. The linear relationship of benzoic acid in the range of 50-250 g/L was better and the limit of detection of benzoic acid in feed was 0.006 g/kg.

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