畜牧与饲料科学 ›› 2009, Vol. 30 ›› Issue (3): 165-165.doi: 10.12160/j.issn.1672-5190.2009.03.092

• 奶业专栏 • 上一篇    下一篇

奶牛布鲁氏菌的PCR鉴定

伊日盖[1] 申之义[1] 李平安[1] 范伟兴[2] 赵妮[2] 张七斤[1]   

  1. [1]内蒙古农业大学,内蒙古呼和浩特010018 [2]中国动物卫生与流行病学中心,山东青岛266032
  • 出版日期:2009-03-20 发布日期:2009-03-20
  • 通讯作者: 伊日盖
  • 作者简介:伊日盖(1985-),女,硕士,主要研究方向为动物传染病诊断与防治。 通讯作者:张七斤(1966-),男,教授,主要研究方向为动物传染病诊断与防治,E-mail:zqj20042004@yahoo.com.cn。

Identification of Brucella in Dairy Cow by PCR

Yirigai, SHEN Zhi-yi, LI Ping-an, FAN Wei-xing, ZHAO Kun, ZHANG Qi-jin (1.Inner Mongolia Agricultural University, Hohhot 010018,China; 2.China Animal Health and Epidemiology Center, Qingdao 266032, China)   

  • Online:2009-03-20 Published:2009-03-20

摘要: 采集内蒙古某地区的可疑感染布鲁氏菌病奶牛的乳样及奶牛流产胎儿的肺、肝、脾等相关病料,分离并培养出11株病原菌,经BCSP31-PCR、VirB8-PCR、多重PCR及AMOS-PCR4种PCR方法鉴定,证实11株病原菌均为布鲁氏菌,并将11株菌定型为牛种5,6,9或3b型。

Abstract: The milk samples and relative samples of lung, liver, spleen etc. in aborted fetus of dairy cow infected with Brucella suspiciously in an area of Inner Mongolia were collected and 11 pathogenic strains were isolate and cultured. 11 pathogenic strains were identified as BruceUa and confirmed as B. abortus biovar 5, 6, 9 or 3b by using 4 kinds of PCR methods as BCSP31-PCR, VirB8-PCR, multiplex PCR and AMOS-PCR.

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