畜牧与饲料科学 ›› 2010, Vol. 31 ›› Issue (3): 93-93.doi: 10.12160/j.issn.1672-5190.2010.03.040

• 遗传与繁育 • 上一篇    下一篇

影响猪卵母细胞玻璃化冷冻保存效果的因素

戴志俊[1,2] 陶勇[2] 刘旭光[2]   

  1. [1]合肥市妇幼保健院生殖医学中心,安徽合肥230001 [2]安徽农业大学动物科技学院,安徽合肥230036
  • 出版日期:2010-03-20 发布日期:2010-03-20
  • 通讯作者: 戴志俊
  • 作者简介:载志俊(1983-),男,硕士,主要研究方向为人类辅助生殖技术。 通讯作者:刘旭光(1964-),男,副教授,硕士,主要研究方向为动物胚胎工程。

Effects of Several Factors on Cryopreservation of Porcine Oocytes by Vitrification

DAI Zhi-jun,TAO Yong,LIU Xu-guang (1.Reproductive Medical Center,Hefei Maternal and Child Health Hospital,Hefei 230001,China;2.College of Animal Science and Technology,Anhui Agricultural University,Hefei 230036,China)   

  • Online:2010-03-20 Published:2010-03-20

摘要: 对卵母细胞进行冷冻保存,可以使卵源不再受到时间和空间的限制,并能为体外受精、核移植和转基因等胚胎工程技术的研究提供充足的原材料。猪卵母细胞中富含脂滴,且对低温十分敏感,因此,猪卵母细胞冷冻保存的研究进展相对较慢。玻璃化冷冻是有效的卵母细胞冷冻方法之一。综述了卵母细胞发育阶段、冷冻载体和冷冻保护剂等因素对猪卵母细胞玻璃化冷冻保存效果的影响。

Abstract: Cryopreservation can make the use of oocytes not constrained by time and space,and the cryopreserved oocytes can be used as materials for in vitro fertilization,nuclear transfer,transgene and other embryo engineering technology.Porcine oocytes are highly sensitive to lower temperatures due to rich lipid droplets in ooplasm.As a result,researches about cryopreservation of porcine oocytes get less progress.Vitrification is one of the effective methods for cryopreservation of oocytes.This paper discusses the effects of developmental stage of oocytes,cryopreservation carriers and cryoprotectant on cryopreservation of porcine oocytes by vitrification.

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