畜牧与饲料科学 ›› 2010, Vol. 31 ›› Issue (11): 6-6.doi: 10.12160/j.issn.1672-5190.2010.11.003

• 基础科学 • 上一篇    下一篇

稀释液中添加多不饱和脂肪酸(PUFA)对绵羊精液冷冻效果的影响

杨健[1] 刘德全[2] 王丽芳[1] 荣威恒[3]   

  1. [1]内蒙古呼和浩特市农牧业局,内蒙古呼和浩特 010020 [2]内蒙古清水河县家畜改良站,内蒙古清水河 012000 [3]内蒙古农牧业科学院,内蒙古呼和浩特 010031
  • 出版日期:2010-11-20 发布日期:2010-11-20
  • 通讯作者: 杨健
  • 作者简介:杨健(1971-),男,高级畜牧师,硕士,主要研究方向为动物遗传育种与繁殖。 通讯作者:荣威恒(1952-),男,研究员,博士生导师,主要研究方向为动物遗传育种与繁殖。

Effects of Diluents Added Polyunsaturated Fatty Acids(PUFA) on Frozen Sheep Semen

YANG Jian,LIU De-quan,WANG Li-fang,RONG Wei-heng(1.Agricultural and Animal Husbandry Bureau of Hohhot City,Hohhot 010020,China;2.Livestock Improvement Station of Qingshuihe County,Qingshuihe 012000,China;3.Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot 010031,China)   

  • Online:2010-11-20 Published:2010-11-20

摘要: 以葡3-3稀释液(葡萄糖-柠檬酸盐-卵黄)为基础,添加富含多不饱和脂肪酸(PUFA),且含较高比例DHA和EPA的深海鱼油作为冷冻绵羊精液的新型添加剂,进而研究PUFA对绵羊精子冷冻—解冻过程优良的保护作用。研究结果表明,绵羊精液冷冻稀释液中添加3%PUFA的处理组,同时添加1%VE(V/V)作为抗氧化剂,经冷冻—解冻对精子的损害较小,解冻后活率和顶体完整率分别比对照组1(0)显著提高6%和10%(P〈0.05),精清中LDH酶(乳酸脱氢酶)活力和GOT酶(谷-草转氨酶)活力比对照组1(0)显著降低65%和15%(P〈0.05)。

Abstract: The novel additive was composed of glucose-citrate-yolk supplemented with deep-seal fish oil which is rich in polyunsaturated fatty acids(PUFA) and contains high-proportion DHA and EPA.This additive was added to frozen sheep semen to study the excellent protection effects of PUFA on sperm in freezing-thawing process.After the freezing-thawing process,the frozen sheep semen in diluents added PUFA at a proportion of 3% and VE at a proportion of 1% were damaged little.After thawing process,viability and acrosomal integrity were 6% and 10% higher than that of the control group 1(0)(P0.05),respectively.The activity of LDH enzyme(Lactate dehydrogenase) and GOT enzyme(glutamate oxaloacetate transaminase) in seminal plasma was 65% and 15% lower than that of the control group 1(0)(P0.05).

中图分类号: