Abstract: To evaluate the effects of different concentrations of 17β-estrodiol （E2） in culture media on in vitro nuclear maturation of porcine oocytes and in vitro development of porcine parthenotes, different concentrations of E2 were added into the medium of porcine ooeytes in vitro maturation. Based solution without adding estradiol to be the control group, the nuclear maturation efficiency, parthenogenetic embryo cleavage rate and blastocyst rate were analyzed. Results showed that the treatment of porcine ooeytes with 1 μg/ mL E2 significantly improved the in vitro maturation, while that treatment of porcine oocytes with 100 μg/mL E2 significantly inhibited the in vitro maturation.