畜牧与饲料科学 ›› 2014, Vol. 35 ›› Issue (2): 4-4.doi: 10.12160/j.issn.1672-5190.2014.02.003

• 基础科学 • 上一篇    下一篇

半胱胺对藏绵羊断奶羔羊瘤胃pH值、总脱氢酶及TVFA的影响

宋天增[1] 冯静[1] 夏晨阳[1] 鲍宇红[2] 冯柯[3] 石国庆[4]   

  1. [1]西藏自治区农牧科学院畜牧兽医研究所,西藏拉萨850009 [2]西藏自治区农牧科学院草业科学研究所,西藏拉萨850000 [3]西藏拉萨市动物疫病预防控制中心,西藏拉萨850009 [4]新疆农垦科学院兵团绵羊繁育生物技术重点实验室,新疆石河子832000
  • 出版日期:2014-02-20 发布日期:2014-02-20
  • 通讯作者: 宋天增
  • 作者简介:宋天增(1980-),男,助理研究员,硕士,主要研究方向为高原畜禽营养与繁殖。 通讯作者:石国庆(1959-),男,研究员,博士生导师,主要研究方向为绵羊繁育。
  • 基金资助:
    863计划项目(2011AAl00307);科技支撑计划(2011BAD28805-1-1);国家自然科学基金(31360540);西藏自治区自然科技基金.

Effects of Cysteamine on pH, Total Dehydrogenase and TVFA of Rumen in Weaned Lambs of Tibetan Sheep

SONG Tian-zeng, FENG Jing, XIA Chen-yang, BAO Yu-hong, FENG Ke, SHI Guo-qing( 1,Institute of Animal Husbandry and Veterinary Medicine, Tibet Academy of Agricultural and Animal Husbandry Sciences, Lhasa 850009, China;2.Institute of Grassland Sciences,Tibet Academy of Agricultural and Animal Husbandry Sciences, Lhasa 850000, China ; 3.Lhasa Prevention and Control Center of Animal Diseases, Lhasa 850009, China; 4.Key Laboratory of Sheep Breeding & Biotechnology in Xinjiang Corps, Xinjiang Academy of Agricultural Reclamation, Shihezi 832000, China)   

  • Online:2014-02-20 Published:2014-02-20

摘要: 试验研究了半胱胺(Cs)对藏绵羊断奶羔羊瘤胃pH值、总脱氢酶及TVFA的影响。将10只(公母各半)80日龄左右、体重平均为(16.00±0.31)kg、装有永久性瘤胃瘘管的藏绵羊断奶羔羊随机分为2组(试验组和对照组),在日粮精料中添加300 mg/(kg·BW)的Cs,隔日添加1次,试验期42 d。正式试验当日及试验第7、14、21、28、35天的7:00、9:00、11:00、13:00、15:00、17:00、19:00、21:00、23:00、1:00、3:00、5:00于颈静脉采血样10 mL,同时采集瘤胃液10 mL,测定瘤胃pH值、总脱氢酶的活性和TVFA。检测结果表明,试验组精料中添加Cs使藏绵羊断奶羔羊瘤胃液pH值升高;血清尿素氮浓度降低,与对照组相比差异极显著(P〈0.01);瘤胃液总脱氢酶活力提高极其明显(P〈0.01);整个试验期内,半胱胺对瘤胃TVFA的影响极显著(P〈0.01)。

Abstract: The effects of cysteamine (Cs) on pH, total dehydrogenase and TVFA in the rumen of weaned lambs of Tibetan Sheep were studied. 10 80-day-old weaned lambs of Tibetan Sheep with loading permanent rumen fistula and the average initial weight of(16.00±0.31)kg were randomly divided into two groups(test group and control group). 300 mg/(kg·BW) of Cs was added in the diet concentrate every two days. The test period lasted for 42 days. 10 mL vein blood samples and tumor gastric juice were collected at 7:00, 9:00, 11:00, 13:00, 15:00, 17:00, 19:00, 21:00, 23:00, 1:00, 3:00 and 5:00 on the 0th, 7th, 14th, 21st, 28th and 35th days. And pH, total dehydrogenase activity and TVFA in the rumen were determined. The results showed that adding Cs in the concentrate in the test group increased pH of rumen juice in the weaned lambs of Tibetan Sheep and decreased the concentration of urea nitrogen in the serum, which had significant difference with control group (P〈0.01). And the activity of total dehydrogenase in gastric juice was significantly enhanced (P〈0.01). In the whole test period, Cs had extremely significant influences on TVFA in the rumen.

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