畜牧与饲料科学 ›› 2014, Vol. 35 ›› Issue (6): 56-56.doi: 10.12160/j.issn.1672-5190.2014.06.023

• 遗传与繁育 • 上一篇    下一篇

微卫星DNA在牦牛亲权鉴定中的应用研究

张浩[1] 安添午[2] 何建文[3] 韩建林[4]   

  1. [1]铜仁职业技术学院农学院,贵州铜仁554300 [2]四川省草原科学研究所,四川成都611731 [3]甘肃农业职业技术学院,甘肃兰州730020 [4]中国农业科学院-国际家畜研究所畜禽牧草遗传资源联合实验室 中国农业科学院北京畜牧兽医研究所,北京100193
  • 出版日期:2014-06-20 发布日期:2014-06-20
  • 通讯作者: 张浩
  • 作者简介:张浩(1981-),男,博士,主要从事动物遗传育种方面的教学和科研工作。

Application of Microsatellite DNA in Paternity Identification of Yak

ZHANG Hao, AN Tian-wu, HE Jian-wen, HAN Jian-lin (1.Agricuhura] College, Tongren Polytechnic College,Tongren 554300,China;2.Sichuan Academy of Grassland Science, Chengdu 611731, China;3. Gansu Agriculture Technology Co]]ege,Lanzhou 730020, China;4.CAAS-ILRI Joint Laboratory on Livestock and Forage Genetic Resources, Institute of Animal Science of CAAS ,Beijing 100193 ,China)   

  • Online:2014-06-20 Published:2014-06-20

摘要: 为了探讨微卫星DNA在牦牛亲权鉴定应用的可行性.选用FAO推荐的17个微卫星座位,检测其在天祝白牦牛群体中的多态性水平,计算这17个微卫星座位用于亲权鉴定的累积排除率。结果表明,17个微卫星座位平均等住基因数为6.235;多态信息含量介于0.191~0.824之间,平均为0.608,为高度多态;一个候选亲本的累积排除概率为0.997,2个候选亲本的累积排除概率为0.99999997,各个微卫星座位零等位基因频率均在0.05以下,微卫星DNA可以应用于牦牛的亲权鉴定。

Abstract: To investigate the feasibility of microsatellite DNA in yak paternity identification applications, 17 microsatellite loci recommended by FAO were selected. The level of polymorphism of these microsatellite loci in Tianzhu White Yak groups was detected,and these 17 microsatellite loci for paternity exclusion probability cumulative was also calculated. Results were as followed: the average allele number of these microsatellite loci was 6.235 ;the polymorphism information content ranged from 0.191 to 0.824, with the average 0.608, showing high polymorphism;a candidate parent cumulative probability of exclusion was 0.997,and the two candidate parent cumulative probability of exclusion was 0.999 999 97;the null allele frequency of each microsatellite loci was below 0.05.Microsatellite DNA could be used for paternity identification in yak.

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