Abstract: Objective] The research aimed to detect cattle and sheep-derived components in the feed of ruminants and animal-derived feed. [Method]The cattle and sheep-derived components in 131 batches of the feed products （premix, concentrate supplement, complete feed, etc.） of ruminants and animal-derived feed （fish meal,poultry meat powder, pork powder, feather meal, etc.） were detected by real-time fluorescence qualitative PCR. [Result] No cattle and sheep-derived component was detected in all samples. [Conclusion] No cattle and sheep-derived component was found in the detected feed products of ruminants and animal-derived feed. The monitoring of cattle and sheep-derived components should still be strengthened.