甘蔗糖蜜培养饲用酵母菌YS-01条件优化的研究

doi:10.12160/j.issn.1672-5190.2017.02.007

畜牧与饲料科学 ›› 2017, Vol. 38 ›› Issue (2): 26-26.doi: 10.12160/j.issn.1672-5190.2017.02.007

甘蔗糖蜜培养饲用酵母菌YS-01条件优化的研究

谢式云[1];马燕[1];金桩[2];易永红[1];陈俊波[3]

[1]罗牛山股份有限公司,海南海口570125 [2]海南罗牛山畜牧有限公司,海南海口570100 [3]海南职业技术学院,海南海口570126

出版日期:2017-02-20 发布日期:2017-02-20
通讯作者: 谢式云
作者简介:谢式云（1973-），女，工程师，主要从事生物化学分析与科研工作。通讯作者：金桩（1967-），男，研究员，博士后，主要研究方向为动物营养及饲料加工。

Optimization of Culture Conditions for Feed Yeast Strain YS-01 with Sugarcane Molasses

XIE Shi-yun1, MA Yan1, JIN Zhuang2, YI Yong-hong1, CHEN Jun-bo3 （1.Luoniushan Co., Ltd., Haikou 570125, China ; 2.Hainan Luoniushan Animal Husbandry Co., Ltd., Haikou 570100, China; 3.Hainan College of Vocation and Technique, Haikou 570126, China）

Online:2017-02-20 Published:2017-02-20

摘要/Abstract

摘要： 通过单因素试验和正交试验对甘蔗糖蜜培养饲用酵母菌YS-01的条件进行了初步优化。结果表明,饲用酵母菌YS-01在处理糖蜜中生长良好,优化的培养条件是：糖蜜浓度为10^0Bx、接种量为8.0%、培养时间为12 h;最佳营养盐配比为：硫酸铵0.10%、尿素0.10%、磷酸二氢钾0.16%、七水硫酸镁0.05%;该条件下OD值达到14.670,活菌数达到2.55×10^-8CFU/m L。

Abstract: In the present study,the single factor experiment and orthogonal experiment were used to optimize the cultivation conditions for feed yeast strain YS-01 with sugarcane molasses. The results revealed that the feed yeast strain showed good growth in the treated molasses; the optimal culture conditions were： molasses concentration was 10^0 Bx, inoculation amount was 8.0%,fermentation duration was 12 h; the optimum concentrations of various nutrients were：（NH4）2SO4 was 0.10%, CO（NH2）2 was 0.10%, KH2PO4 was 0.16%, MgSO4·7H2O was 0.05%. Under this condition, the OD value reached 14.670 and the cell density reached 2.55×10^-8CFU/m L.

中图分类号:

TS249.3 S816.46

谢式云,马燕,金桩,易永红,陈俊波. 甘蔗糖蜜培养饲用酵母菌YS-01条件优化的研究[J]. 畜牧与饲料科学, 2017, 38(2): 26-26.

甘蔗糖蜜培养饲用酵母菌YS-01条件优化的研究

Optimization of Culture Conditions for Feed Yeast Strain YS-01 with Sugarcane Molasses

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