畜牧与饲料科学 ›› 2017, Vol. 38 ›› Issue (10): 5-5.doi: 10.12160/j.issn.1672-5190.2017.10.002
TCDCA对AA模型大鼠成纤维样滑膜细胞中三磷酸肌醇的影响
关恰·桑吉德[1,2];钱英红[3];李培锋[1,2];关红[1,2];何秀玲[1,2];刘博[1,2];曹金山[1,2];毛伟[1,2]
- [1]内蒙古农业大学兽医学院,内蒙古呼和浩特010018;[2]农业部动物临床诊疗技术重点实验室,内蒙古呼和浩特010018;[3]内蒙古自治区农牧业科学院,内蒙古呼和浩特010031
Effect of TCDCA on Concentration of Inositol Trisphosphate in Fibroblast-like Synoviocytes in Rats with Adjuvant Arthritis
Guanqia＊Sangjide12,QIAN Ying-hong3,LI Pei-feng12,GUAN Hong12,HE Xiu-ling12,LIU Bo12,CAO Jin- shan＇,2,MAO Wei1,2（1.College of Veterinary Medicine,Inner Mongolia Agricultural University,Hohhot 010018, China; 2.Key Laboratory of Clinical Diagnosis and Treatment Technology in Animal Disease,Ministry of Agriculture, Hohhot 010018, China; 3.Inner Mongolia Academy of Agricultural and Animal Husbandry Science,Hohhot 010031, China）
摘要： 为了进一步探讨牛磺鹅去氧胆酸（TCDCA）对佐剂性关节炎（adjuvant arthritis,AA）模型大鼠治疗作用的分子机制,以AA模型大鼠成纤维样滑膜细胞（fibroblast-like synoviocytes,FLS）为研究对象,采用酶联免疫吸附技术（enzyme linked immune sorbent assay,ELISA）检测了TCDCA对AA模型大鼠FLS中三磷酸肌醇（inositol trisphosphate,IP3）含量的影响。研究结果显示,与空白对照组相比较,TCDCA作用15 min和60 min时AA模型大鼠FLS中IP3的含量显著升高（P〈0.05）,而当TCDCA作用30 min时AA模型大鼠FLS中IP3的含量极显著升高（P〈0.01）。由此可知,TCDCA能够提高AA模型大鼠FLS中IP3的含量。
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