畜牧与饲料科学 ›› 2017, Vol. 38 ›› Issue (10): 30-30.doi: 10.12160/j.issn.1672-5190.2017.10.009

• 基础科学 • 上一篇    下一篇

雌二醇对奶牛子宫内膜组织中结缔组织生长因子基因表达的影响

李茜如[1,2];钱英红[3];毛伟[1,2];刘博[1,2];张双翼[1,2];沈媛[1,2];曹金山[1,2]   

  1. [1]内蒙古农业大学兽医学院,内蒙古呼和浩特010018;[2]农业部动物疾病临床诊疗技术重点实验室,内蒙古呼和浩特010018;[3]内蒙古自治区农牧业科学院,内蒙古呼和浩特010031
  • 出版日期:2017-10-20 发布日期:2017-10-20
  • 通讯作者: 李茜如
  • 作者简介:李茜如(1992-),女,硕士研究生,主要研究方向为兽医药理学与毒理学.;通讯作者:曹金山(1973-),男,教授,博士(后),博士生导师,主要研究方向为临床药理学.
  • 基金资助:
    国家自然科学基金面上项目(31360806)

Effect of Estrogen on Gene Expression of Connective Tissue Growth Factor in Bovine Endometrium

LI Qian-ru1,2,QIAN Ying-hong3,MAO Wei1,2,LIU Bo1,2,ZHANG Shuang-yi1,2,SHEN Yuan',2,CAO Jin- shani,2(1.College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China; 2.Key Laboratory of Clinical Diagnosis and Treatment Technology in Animal Disease, Ministry of Agriculture, Hohhot 010018, China; 3.Inner Mongolia Academy of Agricultural and Animal Husbandry Science 袁 Hohhot 010031, China)   

  • Online:2017-10-20 Published:2017-10-20

摘要: 观察雌二醇(17β-estraidol,E_2)对奶牛子宫内膜组织中结缔组织生长因子(connective tissue growth factor,CTGF)mRNA表达的影响,并探讨雌激素对奶牛子宫内膜组织修复的机制。体外培养奶牛子宫内膜组织,应用荧光定量PCR技术检测E_2对奶牛子宫内膜组织中CTGF mRNA表达的影响。结果显示,与空白对照组相比较,E_2能够极显著(P〈0.01)地抑制CTGF mRNA的表达。由研究结果可知,E_2能够对奶牛子宫内膜组织中CTGF的基因表达产生调节作用。

Abstract: This study aimed to assess the effect of estrogen (E2) on the mRNA expression of connective tissue growth factor (CTGF) in endometrium of dairy cows, and to uncover the mechanism underling the repairing effect of E2 on bovine endometrium. The mRNA expression of CTGF in the bovine endometrium cultured in vitro was detected by real-time fluorescent quantitative PCR. Compared to the blank control group, the mRNA expression of CTGF in E2 treatment group was significantly decreased (P〈 0.01). In conclusion, our investigation reveals that E2 is able to regulate the gene expression of CTGF in bovine endometrium.

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