Abstract: The present study was designed to screen the optimized extraction process of total flavonoids from Artemisia frigida,a traditional Mongolian medicine.The influences of the factors associated with the extraction efficiency of total flavonoids from Artemisia frigida,including solvents and their corresponding concentrations,solid-liquid ratio of Artemisia frigida and solvents,extraction temperatures,and extraction times,were assessed by using single factor experimental design.The results showed that the optimized extraction process of total flavonoids from Artemisia frigida was as followed:the best extraction solvent was ethanol and its concentration was 60%,the solid-liquid ratio of Artemisia frigida and 60%ethanol was 1∶25,the extraction temperature was 70℃,and the extraction time was 4 h.Our results provide experimental basis for the high-efficiency extraction and clinical use of effective components of Artemisia frigida.