关键词: 冬虫夏草菌;分批发酵;动力学模型

Abstract: [Objective] To establish a kinetic model of batch fermentation of Cordyceps sinensis.[Method] Batch culture of Cordyceps sinensis were performed with a 7 L mechanically stirred fermenter, and the metabolic processes of the cells, extracellular products and substrates were analyzed.Based on Logistic equation and Luedeking-Piret equation, the non-linear fitting and related parameters solving were carried out by using 1stOpt software to establish a fermentation kinetic model of cell growth, extracellular oxalic acid (D-mannitol) synthesis and sugar consumption.[Result] The estimated model parameters obtained by fitting were basically consistent with the fermentation law.The simultaneous fitting R2 of the three separate models was 0.986 5, and R2 of the fitting curves of the cells, products and substrates are 0.971 9, 0.988 0 and 0.991 7, respectively.[Conclusion] The model fits well and can characterize the fermentation kinetic of Cordyceps sinensis.