Abstract: Tributyhin （TBT） is widely distributed in natural environment and is highly toxic. Researches concerning the effect of TBT on animal reproductive function mainly focus on histological variations but barely focus on quantitative analysis. In order to evaluate the effect of TBT on reproductive system of female mice, a low level TBT exposure experiment lasting for 45 days with exposure dosages of 0.2, 2, 20 tLg/（BW＂ kg） was conducted using female Kunming mice as materials. At the end of the exposure experiment, the mice were sacrificed and the ovary, oviduct and uterus samples were collected. To observe the histological variations of the tissue samples, paraffin sections were prepared and stained routinely with hematoxylin and eosin. The thickness of layers of ovary, oviduct and uterus tissues was quantitatively analyzed. The results showed that the changes in quantity of ovarian follicles in different phases were observed： the number of primary follicles showed a decreasing trend and that of the mature follicles was obviously decreased. There were no obvious histological variations in follicles. The thickened oviduct wall was observed, and the histological variations of serous layer and mucosa layer were obvious; the loose arrangement of oviduct internal circular muscle cells was observed. All of the layers of uterus wall showed changes and the most obvious histological variations were found in mucosa layer; the uterus wall became thicker with the increase of TBT exposure dosages. In summary, TBT exposure changed the histological structure of reproductive system in female Kunming mice in a dose-dependent manner.