畜牧与饲料科学 ›› 2018, Vol. 39 ›› Issue (9): 110-112.doi: 10.12160/j.issn.1672-5190.2018.09.028

• 著者文摘 • 上一篇    

兽用药敏纸片的制备及其抑菌效果分析

张浩;郭虹彩;马文奎;曹金元   

  1. 北京农业职业学院,北京102442
  • 收稿日期:2018-06-14 出版日期:2018-08-19 发布日期:2019-08-19
  • 通讯作者: 张浩-北京农业职业学院,北京102442
  • 作者简介:张浩(1966-),女,高级实验师,主要研究方向为兽医临床实验室检测。;通讯作者:曹金元(1962-),男,教授,硕士,主要研究方向为禽病诊断与治疗。
  • 基金资助:
    北京农业职业学院大学生科技创新项目(XY-XK-18-13);2018年北京市农委项目(蛋鸡无抗养殖关键技术示范与推广):北京市农业职业学院技术研发与示范推广项目(XF-FF-18-15).

Preparation and Antimicrobial Effect Evaluation of Antimicrobial Susceptibility Disk for Veterinary Use

ZHANG Hao[1];GUO Hong-cai[1];MA Wen-kui[1];CAO Jin-yuan[1]   

  1. Beijing Vocational College of Agriculture,Beijing 102442,China
  • Received:2018-06-14 Online:2018-08-19 Published:2019-08-19

摘要: [目的]旨在探索常用的兽用药敏纸片的制作方法,节约药敏试验成本。[方法]选取兽医临床上常用的青霉素G、卡那霉素、庆大霉素、氧氟沙星、链霉素、头孢噻呋钠6种抗生素药物,按照治疗量(标记1×)、2倍治疗量(标记2×)和4倍治疗量(标记4×)3个浓度梯度制备药敏纸片。然后利用自制药敏纸片与市售药敏纸片进行药敏试验,对比其抑菌效果。[结果]除头孢噻呋钠外,其余5种常见抗菌药物的2倍治疗量自制药敏片与市售药敏纸片抑菌效果一致。[结论]该试验制备的常用兽用药敏纸片的方法是成功的。

关键词: 兽用药敏纸片;制备方法;抑菌效果

Abstract: [Objective] To explore the preparation method of commonly employed antimicrobial susceptibility disk for veterinary use and to saving the cost of antimicrobial susceptibility test. [ Methods] According to three concentration gradients of treatment dosage (marking l×), twice treatment dosage (marking 2×) and four times treatment dosage (marking 4×), six kinds of antibiotics commonly used in veterinary clinic including penicillin G, kanamycin, gentamicin, ofloxacin, streptomycin and ceftiofur sodium were selected to prepare antimicrobial susceptibility disk. The antimicrobial susceptibility test was carried out with the self-prepared disks and the commercially available disks, respectively, and the antimicrobial effect of them were compared. [ Results] Expect for ceftiofur sodium, the other five kinds of disks containing twice treatment dosage were consistent with the commercially available disks. [Conclusion] The method for preparing antimicrobial susceptibility disk for veterinary use developed in this study was successful.

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