TCDCA对IL-1β刺激下AA大鼠成纤维样滑膜细胞中PGE2分泌的影响

doi:10.12160/j.issn.1672-5190.2021.05.001

畜牧与饲料科学 ›› 2021, Vol. 42 ›› Issue (5): 1-5.doi: 10.12160/j.issn.1672-5190.2021.05.001

• 基础研究 • 下一篇

TCDCA对IL-1β刺激下AA大鼠成纤维样滑膜细胞中PGE₂分泌的影响

姜雪莹^1,2, 钱英红³, 赵俊利³, 张凯^1,2, 段艳¹, 李培锋^1,2, 何秀玲^1,2, 毛伟^1,2, 曹金山^1,2, 郭文瑞^1,2

1.内蒙古农业大学,内蒙古呼和浩特 010018;
2.农业农村部动物疾病诊疗技术重点实验室,内蒙古呼和浩特 010018;
3.内蒙古自治区农牧业科学院,内蒙古呼和浩特 010031

收稿日期:2021-07-18 出版日期:2021-09-30 发布日期:2021-11-25
通讯作者: 郭文瑞（1976—）,男,讲师,博士,主要研究方向为兽医药理学与毒理学。
作者简介:姜雪莹（1997—）,女,硕士研究生,主要研究方向为临床兽医学。
基金资助:
内蒙古自治区自然科学基金项目（2016MS0348）

Effect of Taurochenodeoxycholic Acid on PGE₂ Excretion of Fibroblast-like Synoviocytes in Adjuvant Arthritis Rats with Stimulation of IL-1β

JIANG Xue-ying^1,2, QIAN Ying-hong³, ZHAO Jun-li³, ZHANG Kai^1,2, DUAN Yan¹, LI Pei-feng^1,2, HE Xiu-ling^1,2, MAO Wei^1,2, CAO Jin-shan^1,2, GUO Wen-rui^1,2

1. Inner Mongolia Agricultural University,Hohhot 010018,China;
2. Key Laboratory of Clinical Diagnosis and Treatment Technology in Animal Disease,Ministry of Agriculture and Rural Affairs,Hohhot 010018,China;
3. Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot 010031,China

Received:2021-07-18 Online:2021-09-30 Published:2021-11-25

摘要/Abstract

摘要： [目的] 进一步探讨牛磺鹅去氧胆酸（taurochenodeoxycholic acid,TCDCA）在抗炎免疫方面的潜在调节作用。[方法] 以AA大鼠成纤维样滑膜细胞作为研究对象,采用ELISA方法检测TCDCA和IL-1β作用下AA大鼠成纤维样滑膜细胞上清液中PGE₂的含量,分析TCDCA对IL-1β刺激下AA大鼠成纤维样滑膜细胞中PGE₂分泌情况的影响。[结果] TCDCA能够对IL-1β刺激下AA大鼠纤维样滑膜细胞PGE₂的分泌产生下调作用（P<0.05）。[结论] TCDCA对IL-1β刺激下AA大鼠成纤维样滑膜细胞中PGE₂的分泌具有抑制作用,为TCDCA在兽医临床应用提供依据。

关键词: 牛磺鹅去氧胆酸, 成纤维样滑膜细胞, 前列腺素E₂

Abstract: [Objective] To further assess the potential anti-inflammatory and immunomodulatory roles of taurochenodeoxycholic acid （TCDCA）. [Method] The fibroblast-like synoviocytes （FLS） of adjuvant arthritis （AA） rats were used as experimental research objects. The contents of prostaglandin E₂（PGE₂） in supernatant of FLS co-incubated with TCDCA and IL-1β were determined by ELISA to evaluate the effect of TCDCA on PGE₂ excretion with stimulation of IL-1β. [Result] TCDCA significantly （P<0.05） reduced the PGE₂ excretion level with stimulation of IL-1β in FLS of AA rats. [Conclusion] TCDCA has inhibitory effect on PGE₂ excretion with stimulation of IL-1β in FLS of AA rats, which provides reference for application of TCDCA in veterinary clinic practice.

Key words: taurochenodeoxycholic acid, fibroblast-like synoviocytes, prostaglandin E₂

中图分类号:

S853.74

姜雪莹, 钱英红, 赵俊利, 张凯, 段艳, 李培锋, 何秀玲, 毛伟, 曹金山, 郭文瑞. TCDCA对IL-1β刺激下AA大鼠成纤维样滑膜细胞中PGE₂分泌的影响[J]. 畜牧与饲料科学, 2021, 42(5): 1-5.

JIANG Xue-ying, QIAN Ying-hong, ZHAO Jun-li, ZHANG Kai, DUAN Yan, LI Pei-feng, HE Xiu-ling, MAO Wei, CAO Jin-shan, GUO Wen-rui. Effect of Taurochenodeoxycholic Acid on PGE₂ Excretion of Fibroblast-like Synoviocytes in Adjuvant Arthritis Rats with Stimulation of IL-1β[J]. Animal Husbandry and Feed Science, 2021, 42(5): 1-5.

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TCDCA对IL-1β刺激下AA大鼠成纤维样滑膜细胞中PGE₂分泌的影响

Effect of Taurochenodeoxycholic Acid on PGE₂ Excretion of Fibroblast-like Synoviocytes in Adjuvant Arthritis Rats with Stimulation of IL-1β

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