奶山羊羔羊山羊痘的病理学、血清学及分子生物学诊断

doi:10.12160/j.issn.1672-5190.2022.04.017

畜牧与饲料科学 ›› 2022, Vol. 43 ›› Issue (4): 116-121.doi: 10.12160/j.issn.1672-5190.2022.04.017

奶山羊羔羊山羊痘的病理学、血清学及分子生物学诊断

王娜¹,张帆¹,宋越¹,白帆¹,戴伶俐¹,张月梅¹,李晓燕²,周渊²,王建光³,王根云²,赵世华¹

1.内蒙古自治区农牧业科学院，内蒙古呼和浩特 010031
2.呼和浩特市动物疫病防控中心，内蒙古呼和浩特 010020
3.内蒙古盛健生物科技有限责任公司，内蒙古呼和浩特 011500

收稿日期:2022-04-19 出版日期:2022-07-30 发布日期:2022-07-21
通讯作者: 赵世华（1962—），男，研究员，主要研究方向为动物传染病防控。王根云（1962—），男，高级兽医师，主要研究方向为动物疫病防控。
作者简介:王娜（1989—），女，助理研究员，硕士，主要研究方向为动物传染病防控。
基金资助:
呼和浩特市科技计划项目(2019-农-10);内蒙古自治区科技重大专项(2021ZD0023);内蒙古自治区科技计划项目(2020GG0041);内蒙古自治区农牧业创新基金项目(2020CXJJM06)

Pathological，Serological and Molecular Biological Diagnosis of Goatpox in Dairy Goat Kids

WANG Na¹,ZHANG Fan¹,SONG Yue¹,BAI Fan¹,DAI Ling-li¹,ZHANG Yue-mei¹,LI Xiao-yan²,ZHOU Yuan²,WANG Jian-guang³,WANG Gen-yun²,ZHAO Shi-hua¹

1. Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences，Hohhot 010031，China
2. Hohhot Municipal Prevention and Control Center for Animal Epidemic Disease，Hohhot 010020，China
3. Inner Mongolia Shengjian Biotechnology Co.，Ltd.，Hohhot 011500，China

Received:2022-04-19 Online:2022-07-30 Published:2022-07-21

摘要/Abstract

摘要：

[目的]确定内蒙古某规模化奶山羊养殖场部分引进羔羊发病死亡的原因，为该场加强重点疫病免疫防控提供科学依据。[方法]对出现典型临床症状的发病羊只进行病理解剖学观察，无菌采集肺脏病变样本，制备病理组织切片；无菌采集发病羊只的血液制备血清，采用高敏荧光技术检测血清中的山羊痘病毒抗体；无菌采集发病羊只的肺组织、鼻拭子、眼拭子，利用PCR技术检测山羊痘病毒P32基因，对测序获得的序列进行BLAST比对并进行同源性分析。[结果]剖检可见病羊口腔、鼻腔、喉头、气管黏膜有圆形疹痘；肺部明显水肿，表面存在红色疹痘，切开后呈不透明、灰白色胶冻样；瘤胃、网胃、瓣胃、皱胃黏膜均出现疹痘。病理组织学检查可见肺泡红色深染，间质变宽，腔内存在脱落的上皮细胞，Ⅱ型肺泡上皮细胞增生，并化生为腺泡样状；支气管腔内存在大量的炎性细胞。发病山羊血清经高敏荧光技术检测，判定为山羊痘病毒抗体阳性。采集肺组织（GTPV-YP1）、鼻拭子（GTPV-YP2）、眼拭子（GTPV-YP3）样品各1份，3份样品经P32基因PCR检测均获得983 bp的扩增片段，与预期大小一致；GTPV-YP1、GTPV-YP2样品P32基因序列与山羊痘病毒中国分离株KC951854.1、Oman分离株 MN072621.1 P32基因的同源性为100%，GTPV-YP3与山羊痘病毒中国分离株EF514892.1、HM572329.1、JN596275.1、MG817382.1的同源性为99.0%。[结论]经病理解剖学观察、病理组织学检查、血清学检测以及病原PCR鉴定，确定引起该场引进奶山羊羔羊发病的原因为山羊痘病毒感染。

关键词: 山羊, 山羊痘病毒, 诊断技术

Abstract:

[Objective] To determine the causes of morbidity and mortality of some introduced goat kids in a large-scale dairy goat farm in Inner Mongolia, so as to provide a scientific basis for this farm to strengthen the prevention and control of major epidemic diseases. [Method] The pathological anatomy obaservation of the diseased goat kids with typical clinical signs was made. Pathological sections were prepared using aseptically collected lung samples with lesions. Serum was prepared using aseptically collected blood samples, and the goatpox virus antibody in serum was detected by high-sensitive fluorescence technology. The lung tissues as well as nasal and eye swabs were aseptically gathered, and the P32 gene of goatpox virus was detected by PCR assay and sequenced. The obtained the sequences were subjected to comparision with BLAST and homology analysis. [Result] Pathological autopsy showed that there was round pox in oral, nasal, laryngeal, and tracheal mucosa of the diseased goat kids; the lungs were obviously edematous with red pox on the surface, and were opaque and gray-white jelly like following incision; pox was also visible in the rumen, reticulum, omasum, and abomasum mucosa. Histopathologic examination revealed that the alveoli were intensely stained with red; the alveolar interstitium was widened, and the alveolar cavity contained exfoliated epithelial cells; type Ⅱ alveolar epithelial cells proliferated and had a metaplasia into acinar-like shape; there were massive inflammatory cells in the lumen of bronchi. The goatpox virus antibody in serum of the diseased goat kids was tested positive using high-sensitive fluorescence technology. Lung tissue （GTPV-YP1）, nasal swab （GTPV-YP2） and eye swab （GTPV-YP3） samples were collected, and the P32 gene of a predicted length of 983 bp was all amplified by PCR assay from these samples. The P32 gene sequences of GTPV-YP1 and GTPV-YP2 samples had 100% homology with goatpox virus Chinese isolate KC951854.1 and Oman isolate MN072621.1, and the P32 gene sequence of GTPV-YP3 sample was 99.0% homologous to goatpox virus Chinese isolates EF514892.1, HM572329.1, JN596275.1 and MG817382.1. [Conclusion] The goatpox virus infection was determined as the cause of the illness in the introduced dairy goat kids on the farm by pathological anatomy observation, histological examination, serological detection, and pathogen PCR identification.

Key words: goat, goatpox virus, diagnostic technology

中图分类号:

S858.275.3

王娜, 张帆, 宋越, 白帆, 戴伶俐, 张月梅, 李晓燕, 周渊, 王建光, 王根云, 赵世华. 奶山羊羔羊山羊痘的病理学、血清学及分子生物学诊断[J]. 畜牧与饲料科学, 2022, 43(4): 116-121.

WANG Na, ZHANG Fan, SONG Yue, BAI Fan, DAI Ling-li, ZHANG Yue-mei, LI Xiao-yan, ZHOU Yuan, WANG Jian-guang, WANG Gen-yun, ZHAO Shi-hua. Pathological，Serological and Molecular Biological Diagnosis of Goatpox in Dairy Goat Kids[J]. Animal Husbandry and Feed Science, 2022, 43(4): 116-121.

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参考文献 20

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奶山羊羔羊山羊痘的病理学、血清学及分子生物学诊断

Pathological，Serological and Molecular Biological Diagnosis of Goatpox in Dairy Goat Kids

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