畜牧与饲料科学 ›› 2008, Vol. 29 ›› Issue (5): 24-24.doi: 10.12160/j.issn.1672-5190.2008.05.009

• 试验研究 • 上一篇    下一篇

牛胎儿皮肤成纤维细胞体外培养及其特性研究

周艳喜 曲世磊 李鹏 李燕 刘春霞   

  1. 内蒙古农业大学生物工程学院,内蒙古呼和浩特010018
  • 出版日期:2008-10-20 发布日期:2008-10-20
  • 通讯作者: 周艳喜
  • 作者简介:周艳喜(1984-),男,在读硕士研究生,主要研究方向为动物病理生理。 通讯作者:刘春霞(1978-),女,讲师,主要研究方向为动物发育生物学与胚胎工程,E—mail:liuchunxia-001@163.com.

Studies on the in Vitro Culture and the Characteristics of Bovine Fetus Skin Fibroblasts

ZHOU Yan-xi, QU Shi-lei, LI Peng, LI Yah, Liu Chun-xia (Faculty of Bioengineer , Inner MongoLia Agriculture University , Huhhot 010018, China )   

  • Online:2008-10-20 Published:2008-10-20

摘要: 该试验研究了牛胎儿皮肤成纤维细胞的分离、培养、纯化方法及其生长特征。牛胎儿皮肤细胞贴壁传代2~4次,可成功获得均一稳定的成纤维细胞群体。取传至4代的牛胎儿皮肤成纤维细胞进行细胞计数,并绘制其生长曲线。

Abstract: The study focused on the dissociation, culture, purification and characteristics of bovine fetus skin fibroblasts. Bovine fetus skin fibroblasts had been ohtained by 2-4 times subcultures in vitro. The growth curve had been drawn by cell counting. The 5th, 6th, 7th and 8th passage bovine fetus skin fihrohlasts were manipulated through the technologies of chromosome preparation and microscopic examine of the chromosome change.

中图分类号: