Abstract: A pair of primers was designed according to the Salmonella-specific inv A gene sequence （285 bp;AE008832） published in GenBank.Salmonella in frozen meat were detected by PCR.The results showed that the detection limit of food-borne Salmonella was 103 cfu/mL in saline and 1.98 × 10^3 cfu/mL in artificially polluted broth.After the Salmonella were inoculated into broth at a concentration of 100,101 and 102 cfu/mL,respectively,they could be detected after a 7 h culture,6 h culture and 6 h culture,respectively.