畜牧与饲料科学 ›› 2012, Vol. 33 ›› Issue (8): 1-1.doi: 10.12160/j.issn.1672-5190.2012.08.001

• 基础科学 • 上一篇    下一篇

一株牛源嗜酸性乳杆菌体外抑菌试验研究

高淑琴[1,2] 王庆奎[1,3] 单晓枫[1] 钱爱东[1]   

  1. [1]吉林农业大学动物科学技术学院,占林长春130118 [2]东辽县卫生职业技术学校,吉林东辽136600 [3]辽宁省盘山县高升镇动物卫生监督所,辽宁盘山124123
  • 出版日期:2012-08-20 发布日期:2012-08-20
  • 通讯作者: 高淑琴
  • 作者简介:高淑琴(1965-),女,副主任医师,主要研究方向为微生物学。 通讯作者:钱爱东(1960-),男,教授,博士,博士生导师.主要研究方向为动物微生物与免疫学。
  • 基金资助:
    吉林省畜牧局项目(吉牧科字第200905号).

Experimental Research on Bacteriostasis in vitro of Lactobacillus acidophilus from Calf Origin

GAO Shu-qin', WANG Qing-kui~', SHAN Xiao-fengI, QIAN Ai-dong (1.Animal Science and Technology College, Jilin Agricultural University, Changchun 130118,China;2.Heahh Vocational School of Dongliao County, Dongliao 136600,China;3.Animal Health Supervision Agency of Gaosheng Town, Panshan Country, Liaoning Province, Panshan 124123,China)   

  • Online:2012-08-20 Published:2012-08-20

摘要: 为了验证微生态活菌制剂候选菌株的益生特性,将筛选出的牛源嗜酸性乳杆菌NL0908进行体外抑菌试验.通过共培养和上清液抑菌试验,分析发现,菌株NL0908对病原菌大肠杆菌有较强的抑菌作用。将其上清液进行相应的热处理、蛋白酶处理、中和酸等,结果表明,其抑菌物质主要是由有机酸和抗菌小肽物质共同作用。

Abstract: In order to verify probiotic properlies of candidate strain, put selected NL0908 LactobaciUus acidophilus from calf origin into experiment of bacteriostasis in vitro, through co-culture and supernatant bacteriostatic test, it found that NL0908 strain pathogenic hadantibacterial activity to Escherichia coll. thermal, proteinase treatment and neutralization with supematant, it proved that antibacterial substance may combined action organic acid and mini-peptides of antimicrobial substance.

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