Abstract: The accurate diagnosis of foot-and-mouth disease is essential to control the spread of the disease. With the rapid development of biotechnology, the diagnosis of foot-and-mouth disease is no longer restricted to traditional methods. At present,the commonly used methods are including serological detection which has the widest application range, molecular biological diagnosis and etiological diagnosis. In this paper, a review is given on the principle as well as advantages and disadvantages of the3 above-mentioned diagnostic techniques, and the future development of foot-and-mouth disease diagnostic techniques is prospected.