Abstract: Enterotoxigenic Escherichia coli F18 （ECFI 8 ） with different serotypes is considered to be the causative agent of edema disease and post weaning diarrhea in piglets. The α1 fucosytransferase （FUT1） gene has been identified as a candidate gene for genetic traits showing sensitivity or resistance to F18 mediated adhesion. In the present study, the genetic variation of FUT1 gene was assessed by using PCR-RFLP technique. The genotypes and gene frequency of FUT1 gene in five pig herds in Shanghai, including Duroc （n=77）, Landrace （n=47）, Large White （n=144）, Shennong （n=72） and Meishan （n=69）, were determined. The results showed that 3 genotypes （AA, AG, GG） ofFUT1 gene were observed in hers of Duroc, Large White and Shennong： in Duroc herds, the genotype frequency of AA, AG and GG was 0.16, 0.40 and 0.44, respectively; in Large White herds, the genotype frequency of AA, AG and GG was 0.03, 0.40 and 0.57, respectively; in Shennong herds, the genotype frequency of AA, AG and GG was 0.01, 0.25 and 0.74, respectively. In Landrace herds, the genotypes of AG and GG were found and their genotype frequency was 0.21 and 0.79 respectively, and the genotype of AA （disease-resistant type） was absent. There was only GG genotype observed in Meishan herds.