Abstract: In the study,the contain of chlorophyll and protein from spinach leaf were determined.The result indicated that there was negative correlation between the contain of chlorophyll and protein and ageing;When spinach leaf aged,the contain of chlorophyll decreased,and the decrease in chlorophyll a was higher than that of chlorophyll b;More spinach leaf was serious aging,more the contain of chlorophyll was lower.Meanwhile,the contain of protein decreased,and the activity of protease also increased.