Abstract: The seeding trial on mixed with alfalfa after innoculatedof R.meliloti and Mongolia Agropyron cristatum was made,the analysis and comparation also were taken in morphology,output,quality to explore the effect of increase production.The result showed that the mixed seeding in yield was higher than that of no-innoculation R.meliloti and nitrogen fertilizer application,no-innoculation R.meliloti and no nitrogen fertilizer application achieving 33.91% and 64.89% respectively,it increased the utilization effect for grassland population.The conclusion could be made that the mixed seeding is not only useful for storage and utilization in forage grass,but also improve the feeding value.