Abstract: In recent years,combination of multimedia simulation and training has become an inevitable trend.The development team combined 2D and 3D animation technology with animal surgical operation,took the selection of surgical instruments used in the operation as the carrier,designed the simulation training software on Gastrotomy of Dogs.The software development embodies teaching,art,technology,innovation and practicality.The practice showed that the software was helpful for stimulating students’ interest in learning so as to improve teaching quality.