Abstract: [Objective] To establish a determinative method for macro-elements and trace-mineral elements in mare's milk samples based on full-automatic graphite digestion and inductively coupled plasma-optical emission spectroscopy (ICP-OES) as well as inductively coupled plasma-mass spectrometry (ICP-MS). [Method] The pre-treatment of milk samples was carried out with full-automatic graphite digester; the contents of four macro-elements including kalium, natrium, calcium and phosphorus in the milk samples were determined by using ICP-OES; the standard solutions were prepared with gravimetric method, and the contents of a total of 23 trace-mineral elements including aluminum, arsenic, boron, barium, beryllium, cadmium, cobalt, chromium, copper, iron, lithium, magnesium, manganese, nickel, lead, antimony, tin, strontium, titanium, thallium, vanadium, zinc and selenium were quantitatively analyzed with ICP-MS using rhodium as internal standard; the standard substances for analysis of food bio-components were used to validate the method. [Result] There was a good linear relationship in the range of 0-20 μg/mL for macro-elements and 0-200 ng/mL for trace-elements, and the correlation coefficients of the standard curves were all higher than 0.999; the detection limit for macro-elements by ICP-OES ranged from 0.010 0 to 0.068 8 μg/mL, and that for trace-elements by ICP-MS ranged from 0.001 10 to 1.578 ng/mL; the relative standard deviations (RSDs) of the mineral elements of the standard substances for analysis of food bio-components were less than 11 % (n=6); the determination results were all with the allowable deviation range of the value. [Conclusion] The good linear correlation, detection limit, accuracy and other aspects of the established method can meet the requirements for accurate, efficient and simultaneous determination of mineral elements contents in mare's milk.

Key words: mare's milk, full-automatic graphite digestion, ICP-OES, ICP-MS, standard substances for analysis of food bio-components