羊泰勒虫吉林株的鉴定及系统进化分析

doi:10.12160/j.issn.1672-5190.2020.01.023

Animal Husbandry and Feed Science ›› 2020, Vol. 41 ›› Issue (1): 116-120.doi: 10.12160/j.issn.1672-5190.2020.01.023

Previous Articles Next Articles

Identification and Phylogenetic Analysis of Ovine Theileria Jilin Strains

TIAN Wan-nian, XU Xue-xue, ZHANG Jia-juan

College of Animal Science and Technology, Jilin Agricultural Science and Technology University, Jilin 132101, China

Received:2019-12-06 Online:2020-01-30 Published:2020-03-05

Abstract

Abstract: In order to determine the dominantly prevalent species of ovine Theileria in Yanbian region of Jilin Province, the blood smear microscopy and PCR assay were used to identify the ovine Theileria from the clinical samples, and the phylogenetical analysis of the isolates was carried out subsequently. Blood smear microscopy revealed that round worms were present in the red blood cells of the blood smear. Bioinformatical analysis of the MPSP gene of ovine Theileria Jilin strains demonstrated that the protein encoded by MPSP contains 3 N-glycosylation sites, 14 potential epitopes and a signal peptide structure. The established phylogenetic tree showed that the three clinical isolates in this study were 100% homologous to Theileria luwenshunli (GQ281044) and had a close relationship, indicating that the isolated ovine Theileria were Theileria luwenshuni. The prevalence of Theileria luwenshuni in Jilin Province was observed in this study.

Key words: ovine Theileria, MPSP gene, bioinformatics, phylogenetic tree

CLC Number:

S858.262.729

TIAN Wan-nian, XU Xue-xue, ZHANG Jia-juan. Identification and Phylogenetic Analysis of Ovine Theileria Jilin Strains[J]. Animal Husbandry and Feed Science, 2020, 41(1): 116-120.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://journal30.magtechjournal.com/xmysl/EN/10.12160/j.issn.1672-5190.2020.01.023

https://journal30.magtechjournal.com/xmysl/EN/Y2020/V41/I1/116

References

[1] 朱玉涛,党娜娜,苏布赛,等.新疆部分地区羊泰勒虫病的流行病学调查[J].中国预防兽医学报,2018,40(2):164-166.
[2] 郭淑珍,袁正朴,吴国学,等.甘南藏族自治州羊泰勒虫病流行病学调查[J].中国兽医寄生虫病,2003,11(1):40-41.
[3] 杨辅国,冯泽光,余光海,等.甘南藏族自治州乾安农牧站绵羊泰勒焦虫病报告[J].中国兽医杂志,1958(2):33-37.
[4] 郭淑珍,马登录,牟永娟,等.中国北方地区羊泰勒虫病流行病学调查[J].中国兽医寄生虫病,2005,13(4):15-17.
[5] YIN H, IUO J, SCHNITTGER L, et al.Phylogenetic analysis of Theileria species transmitted by Haemaphysalis qinghaiensis[J].Parasitology Research,2004,92(1):36-42.
[6] 钱年超,贾立军,薛书江,等.牛瑟氏泰勒虫二温式PCR检测方法的建立及应用[J].中国兽医科学,2011,41(11):1150-1153.
[7] 刘娟,刘军龙,刘爱红,等.牛泰勒虫分离鉴定及进化分析[J].中国畜牧兽医,2016,43(4):1050-1057.
[8] 闫亚群,崔艳艳,王晓星,等.羊吕氏泰勒虫PCR检测方法的建立与应用[J].河南农业大学学报,2018,52(2):217-221.
[9] YIN H, LIU Z, GUAN G, et al.Detection and differentiation of Theileria luwenshuni and T. uilenbergi infection in small ruminants by PCR[J].Transboundary and Emerging Diseases,2008,55(5/6):233-237.
[10] 朱玉涛,党娜娜,苏布赛,等.新疆部分地区羊泰勒虫病流行情况调查[J].中国预防兽医学报,2018,40(2):164-166.
[11] 杨菊凤,田慧丽,郭瑞萍,等.山东省媒介蜱类种类鉴定及羊泰勒虫病流行病学调查[J].中国兽医学报,2015,35(6):930-937.
[12] 袁延庆,李有全,刘志杰,等.我国南方地区羊泰勒虫病的流行病学调查[J].中国兽医科学,2013,43(6):551-556.
[13] 宋军科,彭先启,王雪婷,等.羊吕氏泰勒虫陕西分离株的鉴定及遗传进化分析[J].中国兽医学报,2018,38(2):307-310.
[14] 李帅阳,李有全,刘爱红,等.中华泰勒虫MPSP基因的原核表达及反应原性分析[J].中国兽医科学,2014,44(7):690-696.
[15] 钱年超,贾立军,薛书江,等.牛瑟氏泰勒虫MPSP双表位基因的克隆及原核表达[J].中国预防兽医学报,2011,33(10):790-793.

Identification and Phylogenetic Analysis of Ovine Theileria Jilin Strains

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 0

Recommended Articles

Metrics

Comments