Abstract: In order to assess the anticoagulant activity of different parts of Sophora alopecuroides plant,the in vitro clotting time of rabbit blood after co-incubation of alcohol extraction(under ambient temperature)of beans,pods,leaves and shoot apex of Sophora alopecuroides plant collected in autumn was determined by plate blood coagulation method,respectively.The results showed that the alcohol extraction of beans had complete anticoagulant effect;compared to the negative control[(0.9%sodium chloride,(282±11)s],the alcohol extraction of pods[(413±47)s,P<0.01],leaves[(456±32)s,P<0.01]and shoot apex[(317±33)s,P<0.05]significantly extended the clotting time of rabbit blood.The present results indicate that Sophora alopecuroides can prolong the clotting time of blood and has anticoagulant activity,suggesting that this plant is prospected to be developed as a thrombosis-preventive preparation or anticoagulant.