微波水解—氨基酸自动分析仪法测定饲料中色氨酸的含量

doi:10.12160/j.issn.1672-5190.2021.02.004

Abstract

Abstract: [Objective] To develop a rapid determination method for tryptophan in feed by microwave hydrolysis and amino acid automatic analyzer. [Method] The optimum conditions of microwave alkaline hydrolysis were screened by single factor test and orthogonal test, and the tryptophan content in total mixed ration （TMR） of dairy cow was determined by the LCAK06/Na （4.6 mm×150 mm) column of automatic amino acid analyzer. [Result] Hydrolysis temperature was the most important factor affecting tryptophan content in feed. The optimum conditions for determination of tryptophan in feed by microwave alkaline hydrolysis were as follows: hydrolysis temperature 170 ℃, hydrolysis time 30 min, alkali concentration 5 mol/L. The tryptophan content in TMR was 0.14% determined by amino acid automatic analyzer. The linear range of tryptophan was 0.025-0.60 μmol/mL, and the correlation coefficient （r） was 0.999 7. The limit of quantification （LOQ） was 0.05%, the recovery rate of tryptophan was 93%-104%, and the RSD of precision was 3.58%. [Conclusion] The developed method is efficient and accurate, and there is no significant difference in detection results between this method and the conventional hydrolysis method in the national recommended standard of ′Determination of Tryptophan in Feed′ （GB/T 15400-2018）, which has reference value for determination of tryptophan in feed.

Key words: microwave hydrolysis, amino acid automatic analyzer, feed, tryptophan

CLC Number:

S816.17

SONG Jie, WANG Li-fang, YAO Yi-ping, SHI Pei, ZHONG Hua-chen, GUO Chen-yang, ZHANG Xin-xin, HUANG Jie. Determination of Tryptophan in Feed by Microwave Hydrolysis and Amino Acid Automatic Analyzer[J]. Animal Husbandry and Feed Science, 2021, 42(2): 19-25.

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Determination of Tryptophan in Feed by Microwave Hydrolysis and Amino Acid Automatic Analyzer

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