Abstract: [Objective]To domesticate the wild Tupaia belangeri yaoshanenisis in laboratory and to cultivate the experimental animal population.[Methods]The wild Tupaia belangeri yaoshanenisis were kept in a laboratory with controlled temperature,humidity and other conditions.In the initial stage of introduction,the diet was mainly composed of fruits and vegetables,and at the later stage,the diet was gradually transferred to traditional feed.About a month or so,the introduced Tupaia belangeri yaoshanenisis was adaptive to the laboratory feeding environment.Subsequently,the male and female ones who met the requirements of provenance were selected to mate in the same cage,and the ratio of male to female was 1∶1.The weight gain and abdominal palpation were used to judge whether the female one was pregnant or not,and the pregnant ones and their offspring were specially reared and managed.[Results]After one month′s domestication,the survival rate of the wild Tupaia belangeri yaoshanenisis reached up to 96%,and the average weight gain reached 50 g or so.The conception rate,total fetus number,total number born,average litter size,survival rate of birth,and weaning survival rate were 24.53%,12,42,3.5,92.86%,and 42.86%,respectively.[Conclusions]The laboratory domestication of wild Tupaia belangeri yaoshanenisis has been realized,and the propagation of the introduced Tupaia belangeri yaoshanenisis under laboratory condition makes a breakthrough.However,the problems such as low pregnant rate of female ones and low survival rate of their offspring under laboratory domesticative conditions need to be further solved.