Abstract: In order to lay a foundation for further study on the structure and function of CETP, the ORF sequence of human CETP eDNA was cloned and the eukaryotie expression vector was constructed. Total RNA was extracted from human liver tissues and mRNA was purified. Using kit, mRNA was synthesized to cDNA strand through reverse transcription. Primers were designed and the ORF sequence of human CETP cDNA was amplified from synthesized cDNA strand by PCR. And then 1.5 kb eDNA amplified fragment was cloned into pcDNA3.1/myc-His （-）A to form recombinant plasmid vector. Finally, the recombinant plasmid was sequenced after identified. The results showed that the recombinant eukaryotic expression vector contained the complete ORF sequence of CETP eDNA and the sequence was in full accord with the ORF sequence of the reported human CETP eDNA. The eukaryotie expression vector of human cholesteryl ester transfer protein was constructed successfully in this study.