Abstract: The mould and yeast screened from industrial wastewater and domestic sewage were utilized to combine into the compound fungi, the simulation experiment of static treatment was performed on city river sewage, and the dynamic change state of water quality indexes such as total-nitrogen, total-phosphorus and COD during treatment was monitored. The result showed that adding the compound fungi bad a certain impact on water quality, each water quality index all presented decline trend, the largest removal rates of total-P, total-N and COD were 37.4 %, 85.8 % and 53.1%, resp.; but the thalli dosing quantity had no significant influence on the maximum removal rate. This study supplied the basis to utilize microorganism for dealing with the polluted water of city river.