Animal Husbandry and Feed Science ›› 2009, Vol. 30 ›› Issue (4): 3-3.doi: 10.12160/j.issn.1672-5190.2009.04.002

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Fermentation Conditions Optimization of Antagonistic Strain Z-62 against CoUetotrichum gloeosporioides

ZENG Lai-tao, LI Lu-bin, GUO Xiao-jun, LI Shu-na,ZHU Bao-cheng( (1.College of Life Science, Hebei University, Baoding 071002, China; 2. Research Institute of Forestry, Chinese Academy of Forestry, Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100091, China; 3. College of Life Science, Agricuhure University of Hebei, Baoding 071001, China)   

  • Online:2009-04-20 Published:2009-04-20

Abstract: The fermentation conditions of the strain Z-62 was studied via single tactor experiments and orthogonal expenments, and the optimal shaking flask fermentation condition was determined as follows: media composed of 3 % glucose, 3 % soybean peptone, 0.05 % FeSO4·7H2O, 0.05 % KCl, seed age 20 h, initial pH of the media 6.0, media volume 30/250 (mL/mL), inoculum volume 4 % and fermentation time 60 h. In this optimized conditions, the inhibition zone diameter increased by 29.4 % from 20.31 mm. to 26.28 mm.

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