Abstract: To determine the content of flavonoids glycosides in Ginkgo exotesta of both eultivals of fozhi and Dongtinghuang by RP- HPLC.Methods：The separation was performed by using Shim-Paek VP- ODS C18（ 150 min×4.6 ram,5 min）column with a mobile phase consisting of a mixture of methanol- 0.4 % phosphoric acid （50：50） with column temperatur of 30 ℃ and flow rate of 1 mL/min and detective wavelength of 360 nm. The standard curves were made by using quereetin, kaempferol and isorhamnetin as external standard method. The content of flavonoids glycosides in Ginkgo exotesta of both euhivars of fozhi euhivar and Dongtinghuang respectively were 0.134 % and 0.150 %.The content of flavonoids glycosides was different between fozhi and Dongtinghuang, The content of fozhi euhivar was higher than that of Dongtinghuang.