Abstract: The influence of nitrogen, phosphorus, potash and organic fertilizer on the yield of orchardgrass Qiancao NO.4 were evaluated by using quaternary quadratic regression rotation combination design methods. The results showed that the organic fertilizer had the greatest impact on yield of orehardgrass Qiancao NO.4, followed by nitrogen and potash fertilizer, the phosphorus fertilizer has the least impact on the yield; when the yield of orchardgrass Qiancao NO.4 achieved as high as 5 100 kg/667 m2, a total of 3 000 kg organic fertilizer, 30 kg nitrogen fertilizer, 20 kg phosphate fertilizer and 20 kg potash fertilizer were required per 667 m2.