Abstract: [Objective]To assess the roles of G protein-coupled receptor 30（GPR30） in the up-regulatory effects of 17β-estrodiol（E2） on β-defensin-2（SBD-2） expression in ovine oviduct epithelial cells.[Methods]The 2ndpassage ovine oviduct epithelial cells were treated with GPR30 agonist G1（10^-7mol/L） and E2（10^-6mol/L） for 6,12,24 h,respectively.Quantitative real-time PCR（q PCR） was used to evaluate the expression of SBD-2 gene in different observation time points.[Results]Compared with control group,the expression of SBD-2 gene in ovine oviduct epithelial cells was significantly up-regulated（P〈0.01） at the 6thhour of co-treatment of G1 and E2,expressing an obvious time effect.[Conclusion]The GPR30 mediates the membrane receptor signaling pathway involved in SBD-2 gene expression up-regulation in ovine oviduct epithelial cells induced by estrogen.