Abstract: A case of obstinate vomiting in Panthera onca in Nanjing Hongshan Forest Zoo was reported in this paper. The obsolete cage and limited activity space were considered to be associated with the occurrence of obstinate vomiting. The main clinical manifestation of the diseased Panthera onca was the recurring of ＂intaking-vomiting- intaking＂. In the early stage of the disease,the abnormal behavior of the Panthera onca was ignored by the breeders due to the fact that no obvious effects of the behavior on the health of the Panthera onca were observed. Subsequently, a leopard in the same zoo was also found to vomit occasionally. Effective treatment measures were taken to cure the obstinate vomiting of the Panthera onca, and the good curative effect was achieved.