Abstract: Currently, the differences in the development of bio-cleaning technology in SPF chicken production between China and the developed countries still exist. Therefore, further exploration and investigation on this technology are needed. There are two key points in the bio-cleaning technology in SPF chicken production. The first one is to purify the air by using advanced barrier facilities so as to blocking all pathogenic microorganisms from entering into the SPF chicken bio-cleaning room. The second one is to apply strict management measures to prevent the bacteria and viruses that carried by human from entering into the SPF chicken house, and to eliminate the missing or remaining bacteria and viruses in cleanroom by disinfection and sterilization. In this paper,the technology for purification management of SPF chicken house is introduced, so as to provide theoretical basis for the improvement of the purification quality of SPF chickens.