Abstract: In an attempt to investigate the effects of yeast culture addition on development and oviposition of Drosophila melanogaster(D.melanogaster),the developmental time and white pupal weight of D.melanogaster as well as the egg laying numbers of the females reared in the culture medium with addition of 0%,1.5%and 3.0%of yeast culture were determined,respectively,and were statistically assessed.The results showed that the D.melanogaster reared in the culture medium without yeast culture addition died in the 1st larval stage;the developmental time of the larvae was shortened with the elevation of yeast culture addition concentration.When the added concentration of yeast culture was 1.5%,the developmental time of the larvae was similar to the theoretical value,meanwhile,the egg laying numbers of the females were found significantly increased(P<0.05);added concentration of yeast culture had no significant effect on white pupal weight of D.melanogaster(P>0.05).Our results provide a theoretical foundation for the normal culture of D.melanogaster in the labs and for the researches on developmental biology of D.melanogaster.