蓖麻LBD基因家族全基因组鉴定、进化和表达分析

doi:10.12190/j.issn.2096-1197.2021.06.01

北方农业学报 ›› 2021, Vol. 49 ›› Issue (6): 1-14.doi: 10.12190/j.issn.2096-1197.2021.06.01

• 作物遗传育种·种质资源 • 下一篇

蓖麻LBD基因家族全基因组鉴定、进化和表达分析

段强^1,2,3,4,5,6, 何智彪^1,7, 李国瑞^1,2,3,4,5,6, 赵秀平^1,3,4,5,6, 张帅^1,3,4,5,6, 韩雯毓⁷, 陈永胜^1,2,3,4,5,6

1.内蒙古民族大学蓖麻育种国家民委重点实验室,内蒙古通辽 028043;
2.中国科学院长春光学精密机械与物理研究所应用光学国家重点实验室,吉林长春 130000;
3.内蒙古自治区高校蓖麻产业工程技术研究中心,内蒙古通辽 028043;
4.内蒙古自治区蓖麻育种重点实验室,内蒙古通辽 028043;
5.内蒙古自治区蓖麻产业协同创新中心,内蒙古通辽 028043;
6.蓖麻产业技术创新内蒙古自治区工程研究中心,内蒙古通辽 028043;
7.通辽市农牧科学研究所,内蒙古通辽 028043

收稿日期:2021-10-18 出版日期:2021-12-20 发布日期:2022-02-09
通讯作者: 陈永胜（1971—）,男,教授,博士,博士生导师,主要从事植物分子育种的研究工作。
作者简介:段强（1996—）,男,硕士研究生,研究方向为分子生物学。
基金资助:
应用光学国家重点实验室2020年度开放课题（SKLAO2020001A19）; 内蒙古自治区高等教育科研项目（NJZY20121）; 内蒙古自然科学基金项目（2021MS03086）

Genome-wide identification,evolution and expression analysis of LBD gene family in Ricinus communis

DUAN Qiang^1,2,3,4,5,6, HE Zhibiao^1,7, LI Guorui^1,2,3,4,5,6, ZHAO Xiuping^1,3,4,5,6, ZHANG Shuai^1,3,4,5,6, HAN Wenyu⁷, CHEN Yongsheng^1,2,3,4,5,6

1. Key Laboratory of Castor Breeding of the State Ethnic Affairs Commission,Inner Mongolia University for Nationalities,Tongliao 028043,China;
2. State Key Laboratory of Applied Optics,Changchun Institute of Optics,Fine Mechanics and Physics,Chinese Academy of Sciences,Changchun 130000,China;
3. Inner Mongolia Industrial Engineering Research Center of Universities for Castor,Tongliao 028043,China;
4. Inner Mongolia Key Laboratory of Castor Breeding,Tongliao 028043,China;
5. Inner Mongolia Collaborative Innovation Center for Castor Industry,Tongliao 028043,China;
6. Inner Mongolia Engineering Research Center of Industrial Technology Innovation of Castor,Tongliao 028043,China;
7. Tongliao Institute of Agricultural and Animal Husbandry Sciences,Tongliao 028043,China

Received:2021-10-18 Online:2021-12-20 Published:2022-02-09

摘要/Abstract

摘要： 【目的】探究蓖麻LBD转录因子基因家族的组成和表达模式。【方法】通过生物信息学手段,在全基因组水平上筛选和鉴定蓖麻LBD基因家族成员,并对其基因结构、理化性质、蛋白质保守基序、进化选择压力、系统发育树和基因表达量进行分析。【结果】鉴定得出30个蓖麻LBD基因家族成员在进化过程中高度保守,根据LBD结构域和系统发育树分析的特征,将蓖麻LBD基因家族成员分为Class 1和Class 2两类,各有24个和6个。基因结构与蛋白质保守基序分析表明,蓖麻LBD基因家族成员的基因结构与蛋白质保守基序较为简单;亚细胞定位显示,蓖麻LBD基因家族成员全部定位于细胞核,RcLBD12同时也定位于细胞膜。表达量数据分析表明,在蓖麻中,LBD基因家族成员广泛参与叶、茎、根、胚等的发育调控并响应非生物胁迫。【结论】蓖麻LBD基因家族成员在进化历程中出现了大规模片段复制的情况,经历了较强的纯化选择,并且参与调控蓖麻多个组织部位的生长发育过程,其中RcLBD6与RcLBD27可能在蓖麻抵御非生物胁迫的过程中发挥了重要作用。

关键词: 蓖麻, LBD基因家族, 系统进化, 进化选择压力, 表达模式

Abstract: 【Objective】To explore the composition and expression pattern of Ricinus communis LBD transcription factor gene family.【Methods】Bioinformatics was used to screen and identify members of Ricinus communis LBD gene family at the whole genome level,and their gene structure,physical and chemical characteristics,protein conserved motifs,evolutionary selection pressure,phylogenetic tree,and gene expression quantity were analyzed.【Results】There were 30 members of Ricinus communis LBD gene family found,all of which were well conserved throughout evolution. The genes were split into two categories：Class1 and Class2,with 24 and 6 LBD genes,respectively,based on LBD domain and phylogenetic tree analyses. Ricinus communis LBD gene family members′ gene structure and protein conserved motifs were found to be quite simple.Subcellular localization revealed that all members of Ricinus communis LBD gene family were located in the nucleus,with RcLBD12 being in the cell membrane as well. LBD genes are widely involved in the development and regulation of leaves,stems,roots,embryos,and other tissues in Ricinus communis,and had a key role in the resistance to abiotic stress according to expression quantity data.【Conclusion】In the evolutionary process,the LBD gene family members had undergone large-scale fragment replication,selection,and participated in the regulation of the growth and development of many tissues of Ricinus communis. Where the RcLBD6 and RcLBD27 may play an important role in the process of Ricinus communis resistance to abiotic stress.

Key words: Ricinus communis, LBD gene family, Phyletic evolution, Evolutionary selection pressure, Expression pattern

中图分类号:

S565.6

段强, 何智彪, 李国瑞, 赵秀平, 张帅, 韩雯毓, 陈永胜. 蓖麻LBD基因家族全基因组鉴定、进化和表达分析[J]. 北方农业学报, 2021, 49(6): 1-14.

DUAN Qiang, HE Zhibiao, LI Guorui, ZHAO Xiuping, ZHANG Shuai, HAN Wenyu, CHEN Yongsheng. Genome-wide identification,evolution and expression analysis of LBD gene family in Ricinus communis[J]. Journal of Northern Agriculture, 2021, 49(6): 1-14.

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蓖麻LBD基因家族全基因组鉴定、进化和表达分析

Genome-wide identification,evolution and expression analysis of LBD gene family in Ricinus communis

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