低温处理下唐菖蒲休眠球茎中有机酸和可溶性糖的变化规律

doi:10.12190/j.issn.2096-1197.2022.04.13

北方农业学报 ›› 2022, Vol. 50 ›› Issue (4): 96-101.doi: 10.12190/j.issn.2096-1197.2022.04.13

低温处理下唐菖蒲休眠球茎中有机酸和可溶性糖的变化规律

张衡, 张晓瑜, 贺学勤

内蒙古农业大学园艺与植物保护学院,内蒙古呼和浩特 010011

收稿日期:2022-05-23 出版日期:2022-08-20 发布日期:2022-09-30
通讯作者: 贺学勤
作者简介:贺学勤(1971—),女,教授,博士,主要从事观赏植物逆境栽培生理的研究工作。
张衡(1996—),男,硕士研究生,研究方向为观赏植物逆境栽培生理。
基金资助:
内蒙古自然科学基金项目(2021MS03041)

Responses of organic acid and soluble sugar in dormant corms of Gladiolus gandavensis to low temperature

ZHANG Heng, ZHANG Xiaoyu, HE Xueqin

College of Horticulture and Plant Protection,Inner Mongolia Agricultural University,Hohhot 010011,China

Received:2022-05-23 Online:2022-08-20 Published:2022-09-30
Contact: HE Xueqin

摘要/Abstract

摘要：

【目的】明确有机酸代谢和可溶性糖变化对唐菖蒲球茎休眠的影响,为提高唐菖蒲切花精准栽培技术水平提供理论依据。【方法】以唐菖蒲栽培品种“粉友谊”当年生球茎为研究对象,在10 ℃低温下放置不同时间后进行萌发试验,并通过液相色谱法分析唐菖蒲休眠球茎中有机酸、可溶性糖组分与含量的变化规律。【结果】唐菖蒲球茎在低温(10 ℃)处理下,20 d开始休眠解除,60 d休眠完全解除;异亮氨酸(Ile)、赖氨酸(Lys)、脯氨酸(Pro)、γ-氨基丁酸(GABA)、苹果酸(Ma)和柠檬酸(CA)与球茎休眠及休眠解除进程有关,在60 d时这几种有机酸含量均增加,其中,GABA和CA的含量变化最显著(P<0.05);在40 d时唐菖蒲球茎中可溶性糖含量均显著增加(P<0.05),在60 d时葡萄糖、蜜二糖和麦芽糖含量降低;休眠球茎在休眠部分解除时糖酸比值降低,在休眠完全解除时糖酸比值升高。【结论】有机酸和可溶性糖的含量变化与唐菖蒲球茎解除休眠存在紧密联系。

关键词: 唐菖蒲球茎, 休眠解除, 有机酸, 可溶性糖

Abstract:

【Objective】To clarify the effects of organic acid metabolism and soluble sugar changes on the dormancy of Gladiolus gandavensis corms,and to provide the basis for improving the precision cultivation techniques of cut flowers.【Methods】The germination experiment was carried out using current-year corms of the Gladiolus gandavensis cultivar “Pink Friendship”,which had been stored at 10 ℃ for varying lengths of time. The changes in the organic acid and soluble sugar components and contents in the dormant corm of Gladiolus gandavensis were analyzed by liquid chromatography.【Results】Under low temperature(10 ℃)treatment,the dormancy of Gladiolus gandavensis corms began to release after 20 d,and was completely released after 60 d. The processes of dormancy and dormancy release were associated with isoleucine(Ile),lysine(Lys),proline(Pro),aminobutyric acid(GABA),malic acid(MA),and citric acid(CA).The contents of these organic acids increased at 60 d,with the contents of GABA and CA changing most significantly(P<0.05). The content of soluble sugar in Gladiolus gandavensis corm significantly increased at 40 d(P<0.05),while the content of glucose,maloccharide,and maltose decreased at 60 d. The sugar-acid ratio of dormant corms decreased when dormancy was partially released,increased when dormancy was completely released.【Conclusion】The release of dormancy in Gladiolus gandavensis corms was closely correlated with variations in the contents of organic acid and soluble sugar.

Key words: Gladiolus gandavensis corms, Dormancy release, Organic acid, Soluble sugar

中图分类号:

S682.24

张衡, 张晓瑜, 贺学勤. 低温处理下唐菖蒲休眠球茎中有机酸和可溶性糖的变化规律[J]. 北方农业学报, 2022, 50(4): 96-101.

ZHANG Heng, ZHANG Xiaoyu, HE Xueqin. Responses of organic acid and soluble sugar in dormant corms of Gladiolus gandavensis to low temperature[J]. Journal of Northern Agriculture, 2022, 50(4): 96-101.

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低温处理下唐菖蒲休眠球茎中有机酸和可溶性糖的变化规律

Responses of organic acid and soluble sugar in dormant corms of Gladiolus gandavensis to low temperature

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