汉中产淫羊藿不同部位黄酮含量比较分析

doi:10.12190/j.issn.2096-1197.2023.03.05

北方农业学报 ›› 2023, Vol. 51 ›› Issue (3): 39-49.doi: 10.12190/j.issn.2096-1197.2023.03.05

• 作物遗传育种·生理生化 • 上一篇下一篇

汉中产淫羊藿不同部位黄酮含量比较分析

杨洁^1,2, 张秀秀^1,2, 王仕宝^1,2, 何洁³, 黄东萍³, 何志鹏⁴, 张慧^1,2, 房宇^1,2

1.汉中职业技术学院药学院,陕西汉中 723000;
2.汉中职业技术学院秦巴山区药（食）用植物研究所,陕西汉中 723000;
3.汉中市食品药品监督检验检测中心,陕西汉中 723000;
4.陕西国际商贸学院医药学院,陕西西安 712046

收稿日期:2023-03-14 出版日期:2023-06-20 发布日期:2023-08-24
通讯作者: 王仕宝（1982—）,男,副教授,硕士,主要从事药用植物资源开发利用的研究工作。
作者简介:杨洁（1988—）,女,讲师,硕士,主要从事药学专业的教学与科研的研究工作。
基金资助:
陕西省教育厅科学研究计划一般专项（19JK0051,20JK0494）; 2017年中医药公共卫生服务补助专项“全国中药资源普查项目”（财社〔2017〕66号）; 2019年汉中青年科技创新团队课题——秦巴山区药用植物研究团队

Comparative analysis of flavonoids in different parts of Epimedii Folium from Hanzhong

YANG Jie^1,2, ZHANG Xiuxiu^1,2, WANG Shibao^1,2, HE Jie³, HUANG Dongping³, HE Zhipeng⁴, ZHANG Hui^1,2, FANG Yu^1,2

1. School of Pharmacy,Hanzhong Vocational and Technical College,Hanzhong 723000,China;
2. Qinba Mountains Institute of Pharmaceutical（Edible） Botanty,Hanzhong Vocational and Technical College,Hanzhong 723000,China;
3. Hanzhong Center for Food and Drug Supervision,Inspection and Testing,Hanzhong 723000,China;
4. Medical College,Shaanxi Institute of International Trade & Commerce,Xi′an 712046,China

Received:2023-03-14 Online:2023-06-20 Published:2023-08-24

摘要/Abstract

摘要： 【目的】对陕西省汉中市所产淫羊藿不同部位的黄酮含量进行检测比较,为该地区淫羊藿的质量控制提供理论依据。【方法】采集汉中市九县二区,共17批（个）淫羊藿植株地上、地下部分,按照根、茎、叶柄、叶片4个部位,使用高效液相色谱（HPLC）测定朝藿定A1、朝藿定A、朝藿定B、朝藿定C、淫羊藿苷、宝藿苷Ⅰ共6种黄酮类成分,对淫羊藿4个部位的含量差异进行检测分析;并通过 HPLC 对所有样品构建淫羊藿特征指纹图谱,利用中药指纹图谱相似度评价系统判定和指认共有峰,最后绘制17批（个）样品的HPLC指纹图谱。【结果】淫羊藿叶片中6种成分含量均明显高于叶柄和茎;根中6种成分差异较大,其中朝藿定C含量为0.536%~4.712%。根、茎、叶柄和叶片的6种成分总含量分别为0.616%~5.141%、0.006%~0.136%、0.007%~0.271%和0.588%~5.100%;通过中药指纹图谱确定了17批（个）样品中朝藿定A1、朝藿定A、朝藿定B、朝藿定C、淫羊藿苷、宝藿苷Ⅰ 6个共有峰,其根、茎、叶柄和叶片的相似度分别为0.962~1.000、0.791~0.990、0.728~0.996和0.629~0.995。【结论】基于多成分含量测定,明确了汉中产淫羊藿根、茎、叶柄和叶片的质量差异;构建了汉中产淫羊藿HPLC指纹图谱。

关键词: 淫羊藿, 汉中, HPLC, 黄酮含量, 比较分析

Abstract: 【Objective】To compare the flavonoids content in different parts of Epimedii Folium from Hanzhong City,Shaanxi Province,in order to provide theoretical basis for quality control of Epimedii Folium in the region.【Methods】The above-ground and underground parts of 17 batches of Epimedii Folium plants were collected from the nine counties and two districts in Hanzhong City and divided into four parts：roots,stems,petioles,and leaves. High performance liquid chromatography（HPLC） was used to determine the six flavonoids of epimedin A1,epimedin A,epimedin B,epimedin C,icariin,and baohuoside Ⅰ. The content differences of Epimedii Folium in the four parts were detected and analyzed. The Epimedii Folium characteristic fingerprints of all the samples were constructed by HPLC,and the common peaks were determined and identified by the similarity evaluation system of traditional Chinese medicine fingerprints. Finally,HPLC fingerprints of the 17 batches of samples were drawn.【Results】The contents of six components in Epimedii Folium leaves were clearly higher than that of petiole and stem. The six components in the root differed greatly with the content of epimedin C ranging from 0.536% to 4.712%. The total content of the six components in roots,stems,petioles and leaves were 0.616%-5.141%,0.006%-0.136%,0.007%-0.271%,and 0.588%-5.100%,respectively.Six common peaks of epimedin A1,epimedin A,epimedin B,epimedin C,icariin,and baohuoside Ⅰ in the 17 batches of samples were identified by Chinese medicine fingerprints,with similarities of roots,stems,petioles and leaves at 0.962-1.000,0.791-0.990,0.728-0.996,and 0.629-0.995,respectively.【Conclusion】The quality difference of roots,stems,petioles and leaves of Epimedii Folium from Hanzhong was determined based on multi-component content test.The HPLC fingerprint of Epimedii Folium from Hanzhong was established.

Key words: Epimedii Folium, Hanzhong, HPLC, Flavonoids content, Comparative analysis

中图分类号:

R284

杨洁, 张秀秀, 王仕宝, 何洁, 黄东萍, 何志鹏, 张慧, 房宇. 汉中产淫羊藿不同部位黄酮含量比较分析[J]. 北方农业学报, 2023, 51(3): 39-49.

YANG Jie, ZHANG Xiuxiu, WANG Shibao, HE Jie, HUANG Dongping, HE Zhipeng, ZHANG Hui, FANG Yu. Comparative analysis of flavonoids in different parts of Epimedii Folium from Hanzhong[J]. Journal of Northern Agriculture, 2023, 51(3): 39-49.

参考文献

[1] 黄岩,张钧.淫羊藿的发展现状、存在问题及建议[J].河南农业,2022(28):55.
[2] 李铖,崔毓桂,覃莲菊,等.淫羊藿(苷)对男性生殖系统的作用和机制[J].国际生殖健康/计划生育杂志,2022,41(3):236-239.
[3] 刘摇摇,刘湘宁,田银平,等.淫羊藿苷对骨骼系统药理作用机制的研究进展[J].中药新药与临床药理,2020,31(12):1516-1520.
[4] 马震东,黄淑芸,黄一兴,等.淫羊藿治疗骨质疏松的网络药理学及分子对接技术研究[J].牡丹江医学院学报,2022,43(5):35-40.
[5] 岑延祐,刘煜德,张洪冠,等.淫羊藿总黄酮对雄性自发性高血压病大鼠血压、性功能的作用及机制研究[J].中国中西医结合杂志,2022,42(6):716-721.
[6] 金久楚,卢敏,邝高艳,等.淫羊藿作用于膝骨关节炎的分子机制研究[J].中医药临床杂志,2022,34(10):1890-1896.
[7] 史阔豪,张光财,武康雄,等.4种淫羊藿黄酮苷类主要成分体外药理活性比较[J].陕西科技大学学报,2021,39(2):56-61.
[8] 龚华乾,高敏,柴艺汇,等.淫羊藿化学成分与药理作用研究进展[J].湖北民族大学学报(医学版),2021,38(4):75-78.
[9] CHO W K,MA J Y.Antiviral activity of Epimedium koreanum Nakai water extract against influenza viruses[J].Biomedicine & Pharmacotherapy,2022,146:112581.
[10] 齐芸,郑永先.淫羊藿苷抗肿瘤作用机制研究进展[J].中医药导报,2022,28(8):153-157.
[11] 华辞怡,史有阳,谢颖,等.淫羊藿有效成分治疗乳腺癌作用机制的研究进展[J].中草药,2022,53(20):6593-6600.
[12] 周晓洁,姚辛敏,周妍妍.淫羊藿的药理作用研究进展[J].中医药学报,2022,50(11):112-115.
[13] 张续蓝,王婷,郑杰,等.从AMPK-mTOR信号途径研究淫羊藿总黄酮对衰老大鼠皮层自噬的调节作用[J].中国药理学通报,2020,36(10):1445-1449.
[14] 国家药典委员会.中华人民共和国药典:一部[S].北京:中国医药科技出版社,2020:340-342.
[15] 李子豪,柯仲成,封亮,等.宝藿苷Ⅰ的制备方法及药理作用研究进展[J].中国中药杂志,2018,43(17):3444-3450.
[16] 王仕颖,孙崇崇,郑丹雯,等.宝藿苷Ⅰ对MPP+诱导SH-SY5Y细胞损伤保护作用[J].青岛大学学报(医学版),2022,58(3):337-340.
[17] 詹扬,韦英杰,孙娥,等.二维斑马鱼模型联合色谱技术评价朝藿定A及其代谢物宝藿苷Ⅰ抗骨质疏松活性[J].药学学报,2014,49(6):932-937.
[18] 徐忠坤,殷洪梅,李芳,等.淫羊藿总黄酮胶囊中黄酮类成分含量测定及抗骨质疏松活性研究[J].中国中药杂志,2018,43(15):3140-3144.
[19] 白洋,王英哲,姜大成,等.不同产地朝鲜淫羊藿的土壤因子与药材质量相关性分析[J].时珍国医国药,2022,33(1):215-218.
[20] 雷荣,汪卓逸,汪永平,等.遮阴度对“黔藿1号”箭叶淫羊藿生长及产质量的影响[J].中药材,2021,44(11):2501-2505.
[21] 李仁清,田淑云,李风琴,等.4种淫羊藿产量与质量的动态变化及最佳采收期研究[J].时珍国医国药,2022,33(6):1456-1462.
[22] 王燕萍,贾旭森,王艳,等.不同干燥方式对淫羊藿黄酮类成分含量及抗氧化活性影响[J].中草药,2021,52(14):4193-4200.
[23] ZHAO Y D,CHEN S F,WANG Y D,et al.Effect of drying processes on prenylflavonoid content and antioxidant activity of Epimedium koreanum Nakai[J].Journal of Food and Drug Analysis,2018,26(2):796-806.
[24] 许婷,黄萌萌,李瑞云,等.多基原淫羊藿不同部位质量评价研究[J].中草药,2020,51(1):190-196.
[25] 王仕宝,蔡艳妮,贾慧梅,等.HPLC法测定不同来源重楼药材中7种甾体皂苷的含量[J].西北药学杂志,2017,32(2):133-138.
[26] 滕熙超,张宇宁,张永刚,等.指纹图谱评价和多元统计分析不同产地朝鲜淫羊藿药材质量[J/OL].中华中医药学刊,2022:1-9.(2022-10-09).http://kns.cnki.net/kcms/detail/21.1546.R.20221009.0903.002.html.

汉中产淫羊藿不同部位黄酮含量比较分析

Comparative analysis of flavonoids in different parts of Epimedii Folium from Hanzhong

PDF (PC)

赞

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 2

Metrics

本文评价

推荐阅读 0

[1]	成建宏苏二虎李银换李莲香冯智霞. 玉米不同轴色、粒色品种营养成分比较研究[J]. 北方农业学报, 2013, (6): 38-38.
[2]	智小青张艳芳. 乌兰察布盟韭菜无公害生产技术[J]. 北方农业学报, 2003, (B12): 39-39.