跨界小RNA参与植物病原菌互作的研究进展

doi:10.3969/j.issn.2096-1197.2019.06.11

摘要/Abstract

摘要： 植物小RNA介导的RNA干扰在植物响应病原菌侵染的免疫反应中发挥重要作用。研究发现,小RNA（sRNAs）可以跨界转运,参与植物免疫和病原菌的致病过程,这种突破物种界限,转运到寄主或微生物体内沉默靶基因的现象,称为跨界RNA干扰（Cross-kingdom RNAi）。跨界 RNA干扰技术的应用为当前植物保护和抗病育种提供了新的方向。文章综述了跨界sRNAs的合成路径、转运机制及其在植物免疫和病原菌毒力方面的研究进展,以期为跨界 RNA干扰技术在植物病害防控领域的应用提供参考信息。

关键词: 跨界sRNAs, RNA干扰技术, 病原菌, 植物免疫

Abstract: RNA interference mediated by small RNAs plays an important role in plant immune response to pathogen infection.Researches have shown that small RNAs can be transported to silent target genes across borders and participate in plant immunity and pathogen′s pathogenic process,which is called Cross-kingdom RNAi.The application of Cross-kingdom RNAi technology provides a new direction for plant protection and disease resistance breeding.In this paper,the synthesis pathway,the action mechanism,the transport mechanism,and the research progress in plant immunity and pathogen virulence of Cross-kingdom small RNA are reviewed in order to provide reference information for the application of Cross-kingdom RNAi technology in plant disease prevention and control.

Key words: Cross-kingdom sRNAs, Small RNA interference, Pathogen, Plant immune

中图分类号:

S432.2

郝丽芬, 燕孟娇, 房永雨, 宋培玲, 皇甫海燕, 郭晨, 杨永青, 贾晓清, 皇甫九茹, 李子钦. 跨界小RNA参与植物病原菌互作的研究进展[J]. 北方农业学报, 2019, 47(6): 62-67.

HAO Lifen, YAN Mengjiao, FANG Yongyu, SONG Peiling, HUANGFU Haiyan, GUO Chen, YANG Yongqing, JIA Xiaoqing, HUANGFU Jiuru, LI Ziqin. Advances in the study of Cross-kingdom sRNAs involved in the plant-pathogen interaction[J]. Journal of Northern Agriculture, 2019, 47(6): 62-67.

参考文献

[1] HUANG C Y,WANG H,HU P,et al.Small RNAs—Big players in plant-microbe interactions[J].Cell Host & Microbe,2019,26(2):173-182.
[2] KATIYAR-AGARWAL S,MORGAN R,DAHLBECK D,et al.A pathogen-inducible endogenous siRNA in plant immunity[J].Proc Natl Acad Sci USA,2006,103(47):18002-18007.
[3] BOCCARA M,SARAZIN A,THIÉBEAULD O,et al.The Arabidopsis miR472-RDR6 silencing pathway modulates PAMP- and effector-triggered immunity through the post-transcriptional control of disease resistance genes[J].PLoS Pathog,2014,10:e1003883.
[4] LI F,PIGNATTA D,BENDIX C,et al.MicroRNA regulation of plant innate immune receptors[J].Proc Natl Acad Sci USA,2012,109(5):1790-1795.
[5] DENG Y,ZHAI K,XIE Z,et al.Epigenetic regulation of antagonistic receptors confers rice blast resistance with yield balance[J].Science,2017,355(6328):962-965.
[6] WANG H,SEO J K,GAO S,et al.Silencing of AtRAP,a target gene of a bacteria-induced small RNA,triggers antibacterial defense responses through activation of LSU2 and down-regulation of GLK1[J].New Phytol,2017,215:1144-1155.
[7] WEIBERG A,WANG M,LIN F M,et al.Fungal small RNA ssuppress plant immunity by hijacking host RNA interference pathways[J].Science,2013,342(6154):118-123.
[8] KATIYAR-AGARWAL S,JIN H.Role of small RNAs in host-microbe interactions[J].Annual Review of Phytopathology,2010,48(1):225-246.
[9] BORSANI O,ZHU J,VERSLUES P E,et al.Endogenous siRNAs derived from a pair of natural cis-antisense transcripts regulate salt tolerance in Arabidopsis[J].Cell,2005,123(7):1279-1291.
[10] VAUCHERET H.Post-transcriptional small RNA pathways in plants:mechanisms and regulations[J].Genes Dev,2006,20(7):759-771.
[11] 吴婧,邢晶晶,王梦婷,等.植物体内的非经典蛋白分泌途径[J].电子显微学报,2018,37(2):160-170.
[12] AN Q,HUCKELHOVEN R,KOGEL K H,et al.Multivesicular bodies participate in a cell wall-associated defence response in barley leaves attacked by the pathogenic powdery mildew fungus[J].Cell Microbiol,2006,8(6):1009-1019.
[13] CAI Q,HE B Y,JIN H L.A safe ride in extracellular vesicles - small RNA trafficking between plant hosts and pathogens[J].Current Opinion in Plant Biology,2019,52:140-148.
[14] HALPERIN W,JENSEN W A.Ultrastructural changes during growth and embryogenesis in carrot cell cultures[J].J Ultrastruct Res,1967,18(3):428-443.
[15] REGENTE M,CORTI-MONZON G,et al.Vesicular fractions of sunflower apoplastic fluids are associated with potential exosome marker proteins[J].FEBS Lett,2009,583(20):3363-3366.
[16] COLOMBO M,RAPOSO G,THEÉRY C.Biogenesis,secretion,and intercellular interactions of exosomes and other extracellular vesicles[J].Annu Rev Cell Dev Biol,2014,30:255-289.
[17] LIPKA U,FUCHS R,LIPKA V.Arabidopsis non-host resistance to powdery mildews[J].Curr Opin Plant Biol,2008,11(4):404-411.
[18] CAI Q,QIAO L,WANG M,et al.Plants send small RNAs in extracellular vesicles to fungal pathogen to silence virulence genes[J].Science,2018,360,(6393):1126-1129.
[19] HOU Y,ZHAI Y,FENG L,et al.A phytophthora Effector suppresses trans-kingdom RNA Ⅰ to promote disease susceptibility[J].Cell Host Microbe,2018,25:153-165.
[20] NAVARROET A L,NAVARRO L,DUNOYER P,et al.A plant miRNA contributes to antibacterial resistance by repressing auxin signaling[J].Science,2006,312(5772):436-439.
[21] ZHANG T,ZHAO Y L,ZHAO J H,et al.Cotton plants export microRNAs to inhibit virulence gene expression in a fungal pathogen[J].Nat Plants,2016,2:16153.
[22] XU J,WANG X,LI Y,et al.Host-induced gene silencing of a regulator of G protein signalling gene (VdRGS1) confers resistance to Verticillium wilt in cotton[J].Plant Biotechnol J,2018,16(9):1629-1643.
[23] FILIATRAULT M J,STODGHILL P V,BRONSTEIN P A,et al.Transcriptome analysis of Pseudomonas syringae identifies new genes,noncoding RNAs,and antisense activity[J].J Bacteriol,2010,192(9):2359-2372.
[24] LIANG H,ZHAO Y T,ZHANG J Q,et al.Identification and functional characterization of small non-coding RNAs in Xanthomonas oryzae pathovar oryzae[J].BMC Genomics,2011,12:87.
[25] WILMS I,OVERLOPER A,NOWROUSIAN M,et al.Deep sequencing uncovers numerous small RNAs on all four replicons of the plant pathogen Agrobacterium tumefaciens[J].RNA Biol,2012,9(4):446-457.
[26] RAMAN V,SIMON S A,ROMAG A,et al.Physiological stressors and invasive plant infections alter the small RNA transcriptome of the rice blast fungus,Magnapor the oryzae[J].BMC Genomics,2013,14(1):326.
[27] WANG M,WEIBERG A,LIN F M,et al.Bidirectional cross-kingdom RNAi and fungal uptake of external RNAs confer plant protection[J].Nat Plants,2016,2:16151.
[28] WANG B,SUN Y F,SONG N,et al.Puccinia striiformis f.sp. tritici microRNA-like RNA 1(Pst-milR1),an important pathogenicity factor of Pst,impairs wheat resistance to Pst by suppressing the wheat pathogenesis-related 2 gene[J].New Phytol,2017,215(1):338-350.
[29] ZHU C,LIU T,CHANG Y N,et al.Small RNA functions as a trafficking effector in plant immunity[J].Int J Mol Sci,2019,9:20(11):2816.
[30] 张杰,董莎萌,王伟,等.植物免疫研究与抗病虫绿色防控:进展、机遇与挑战[J].中国科学:生命科学,2019,49(11):1479-1507.
[31] ZHANG T,JIN Y,ZHAO J H,et al.Host-induced gene silencing of the target gene in fungal cells confers effective resistance to the cotton wilt disease pathogen Verticillium dahliae[J].Mol Plant,2016,9:939-942.
[32] GOVINDARAJULU M,EPSTEIN L,WROBLEWSKI T,et al.Host-induced gene silencing inhibits the biotrophic pathogen causing downy mildew of lettuce[J].Plant Biotechnology Journal,2015,13(7):875-883.
[33] CHEN W,KASTNER C,NOWARA D,et al.Host-induced silencing of Fusarium culmorum,genes protects wheat from infection[J].Journal of Experimental Botany,2016,67(17):4979-4991.
[34] SKOMINSKA-DURDASIAK K M,KOLLERS S,KORZUN V,et al.Association mapping of wheat Fusarium head blight resistance-related regions using a candidate-gene approach and their verification in a biparental population[J].Theoretical and Applied Genetics,2019.https://doi.org/10.1007/s00122-019-03463-52.
[35] ZHU X,QI T,YANG Q,et al.Host-induced gene silencing of the MAPKK gene PsFUZ7 confers stable resistance to wheat stripe rust[J].Plant Physiology,2017,175(4):1853-1863.