不同耐盐性菜用大豆与大豆根瘤菌的共生匹配性研究

doi:10.3969/j.issn.2096-1197.2019.06.16

北方农业学报 ›› 2019, Vol. 47 ›› Issue (6): 90-95.doi: 10.3969/j.issn.2096-1197.2019.06.16

不同耐盐性菜用大豆与大豆根瘤菌的共生匹配性研究

代重阳, 孙继鑫, 王聪

内蒙古民族大学农学院,内蒙古通辽 028000

收稿日期:2019-10-15 发布日期:2020-01-19
通讯作者: 王聪（1968—）,男,教授,博士,主要从事蔬菜生理和生物技术研究工作。
作者简介:代重阳（1994—）,男,硕士研究生,研究方向为逆境生理。
基金资助:
国家自然科学基金项目（31260472）

Symbiotic matching between soybean rhizobium and vegetable soybean with different salt tolerance

DAI Chongyang, SUN Jixin, WANG Cong

College of Agriculture,Inner Mongolia University for Nationalities,Tongliao 028000,China

Received:2019-10-15 Published:2020-01-19

摘要/Abstract

摘要： 为筛选与不同耐盐性菜用大豆结瘤固氮能力较强的大豆根瘤菌,以蛭石作基质,采用人工气候箱栽培,选用10株大豆根瘤菌（NGR234、HH103、B16、N18、USDA110、USDA122、CB1809、ACCC15067、AS1.826、AS1.909）及菜用大豆耐盐品种绿领八号、盐敏感品种日本青为试验材料进行共生匹配性研究。结果显示：绿领八号-USDA122组合的地上部生物量与接种USDA110的差异不显著,根瘤鲜重与接种N18、USDA110及AS1.826的差异不显著,植株全氮量与接种HH103的差异不显著,但均显著高于其他组合。日本青-HH103组合的根瘤数、根瘤鲜重、根瘤固氮酶活性及植株全氮量均显著高于其余组合,但其地上部生物量与接种USDA110的差异不显著。为更准确地评价各菌种与两菜用大豆品种的结瘤固氮能力,本研究采用了综合评价方法,结果表明各菌种与绿领八号共生固氮能力由强到弱依次是：USDA122>AS1.826>HH103、USDA110>N18>AS1.909>ACCC15067、NGR234>B16、CB1809,与日本青共生固氮能力由强到弱依次是：HH103>USDA110>N18>USDA122、NGR234>AS1.909>B16>ACCC15067、AS1.8>CB1809。上述结果表明,不同大豆根瘤菌之间结瘤固氮能力存在显著差异,耐盐品种绿领八号与USDA122的结瘤固氮能力较强,盐敏感品种日本青与HH103的结瘤固氮能力较强,综合评价方法可为大豆与根瘤菌共生匹配性研究提供参考。

关键词: 菜用大豆, 大豆根瘤菌, 结瘤固氮

Abstract: In order to screen soybean rhizobium with strong nitrogen fixation ability for vegetable soybean with different salt tolerance,ten Soybean Rhizobium strains （NGR234,HH103,B16,N18,USDA110,USDA122,CB1809,ACCC15067,AS1.826,and AS1.909）were selected and used in the study with vegetable soybean salt-tolerant varieties LvLingBaHao Symbiotic matching of salt-sensitive cultivar RiBenQing.The results showed that there was no significant difference in the overground biomass between LvLingBaHao-USDA122 combination and the inoculation of USDA110,the fresh weight of nodules had no significant difference with the inoculation of N18,USDA110 and AS1.826,and the total nitrogen content of plants had no significant difference with the inoculation of HH103,but they were significantly higher than other combinations.The number of nodules,the fresh weight of nodules,the activity of nodules dinitrogenase and the total nitrogen content in plants of RiBenQing-HH103 combination were significantly higher than those of other combinations,but there was no significant difference in the overground biomass between RiBenQing-HH103 combination and USDA110 inoculation.In order to accurately evaluate the nitrogen fixation ability of each strain and two vegetable soybean varieties,a comprehensive evaluation method was adopted in this study.The results showed that the symbiotic nitrogen fixation ability of each strain and LvLingBaHao from strong to weak was：USDA122>AS1.826>H103,USDA110>N18>AS1.909>ACCC15067,NGR234>B16,CB1809,the symbiotic nitrogen fixation ability with RiBenQing was HH103>USDA110>N18>USDA122,NGR234>AS1.909>B16>ACCC15067,AS1.8>CB1809.Those results showed that there were significant differences in nodule and nitrogen fixation ability among different Soybean Rhizobium strains.The salt-tolerant cultivar LvLingBaHao and USDA122 had stronger nodule and nitrogen fixation ability,while the salt-sensitive cultivar RiBenQing and HH103 had stronger nodule and nitrogen fixation ability. In addition,the comprehensive evaluation method introduced in this study can also provide a reference for the study of symbiotic matching between soybean and rhizobium.

Key words: Vegetable soybean, Soybean rhizobium, Nitrogen fixation

中图分类号:

S154.3

代重阳, 孙继鑫, 王聪. 不同耐盐性菜用大豆与大豆根瘤菌的共生匹配性研究[J]. 北方农业学报, 2019, 47(6): 90-95.

DAI Chongyang, SUN Jixin, WANG Cong. Symbiotic matching between soybean rhizobium and vegetable soybean with different salt tolerance[J]. Journal of Northern Agriculture, 2019, 47(6): 90-95.

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不同耐盐性菜用大豆与大豆根瘤菌的共生匹配性研究

Symbiotic matching between soybean rhizobium and vegetable soybean with different salt tolerance

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