北方农业学报 ›› 2008, Vol. ›› Issue (3): 54-54.

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菊苣组织培养及无性系建立的研究

高琳娜 陈鹏彦 姜长阳   

  1. 辽宁师范大学生命科学学院,辽宁大连116029
  • 出版日期:2008-06-20 发布日期:2008-06-20
  • 通讯作者: 高琳娜
  • 作者简介:高琳娜(1984-),女,辽宁鞍山人,辽宁师范大学硕士研究生,从事植物生物技术研究。

In Vitro Culture of Chicory and its Clones Establishment

GAO Lin-na (College of Life Science, Liaoning Normal University, Dalian 116029,China)   

  • Online:2008-06-20 Published:2008-06-20

摘要: 以具有有利变异性状的菊苣叶柄为材料,进行了组织培养及无性系建立的研究。结果证明:MS+BA0.4mg/L+2,4-D丁酯1.6mg/L是诱导叶柄形成具有分化能力愈伤组织的理想培养基:1/2MS+AgNO3 20.5mg/L+BA0.3mg/L+NAA0.1mg/L是愈伤组织和不定芽分化的理想培养基;1/3MS+IAA0.2~0.4mg/L是变异菊苣不定芽生根的理想培养基;炉灰渣是菊苣试管苗移栽的理想基质。

Abstract: A study on the technique of tissue culture and establishment of asexual system of Cichorium intybus was conducted. The results indicated that the ideal medium for induction and differentiation of buds from stem segments are MS+BA0.4mg/L+ 2,4-D1.6mg/L and 1/2 MS+ AgNO3 20.5mg/L+BA0.3mg/L+NAA0.1mg/L, respectively, The medium of 1/3MS+IAA0.2-0.4 mg/L is suitable for root growth.

中图分类号: 

  • S688.4 Q943.1