Abstract: Objective：Ruta graveolens is a kind of medicinal plants,the output of its stem is very high.Rodenticide made from Ruta graveolens will provide theoretical basis for the production and application of rodenticide.Methods：The stem tissue of Ruta graveolens was used to produce poisonous bait of high,medium,and low concentration,respectively.Then poisonous baits were used to feed male mice and changes of body weight,testis mass,small intestine mass as well as infertility rate for female mice copulated with male mice fed with poisonous bait were observed.Results：In comparison with the control group,mice fed with poisonous bait exhibited marked reduction in body weight,testis mass as well as small intestine mass corresponding with the increase of infertility rate.Conclusion：Poisonous bait made from Ruta graveolens could effectively restrain the proliferation of mice.