堇菜无性系建立的研究

北方农业学报 ›› 2012, Vol. ›› Issue (4): 33-33.

堇菜无性系建立的研究

杨丹刘姝孙靖靓宁淑香姜长阳

f辽宁师范大学生命科学学院,辽宁大连116029

出版日期:2012-08-20 发布日期:2012-08-20
通讯作者: 杨丹
作者简介:通讯作者：姜长阳（1953-），男，辽宁大连人，教授，从事植物技术研究与教学工作。
基金资助:
基金项目：辽宁省高等教育教学改革资助项目（20090304）;辽宁师范大学教学改革资助项目（LSJG：20090108）

Study on Common Violet Clones Establishment

YANG Dan （College of Life Science,Liaoning Normal University,Dalian 116029,China）

Online:2012-08-20 Published:2012-08-20

摘要/Abstract

摘要： 为满足栽培对种苗的需求，以子叶为材料，采用组织培养的方法，对堇菜进行了愈伤组织诱导与分化、不定芽的分化、不定芽生根与试管苗生根继代增殖、试管苗的移栽和定植的研究，建立起堇菜的无性系。结果证明：MS＋BA0．4mg／L＋2，4-D丁酯2．5mg／L是子叶愈伤组织的诱导培养和继代增殖培养的理想培养基：MS＋NAA0．1mg／L＋BA0．6mg／L是愈伤组织分化培养和不定芽分化增殖继代培养的理想培养基：1／4MS＋ABT2号2mg／L＋NAA0．3mg／L是不定芽生根和试管苗生根继代增殖培养的理想培养基；试管苗移栽成活率为91．9％，定植成活率为99．1％。定植成活的试管苗生长旺盛，并保持了堇菜的所有植物学特征。

Abstract: The aim was to meet the need for cultivation of germchit.By using tissue culture methods,the cotyledons of Viola verecunda were used as material to do the research on callus induction and differentiation,differentiation and rooting of adventitious buds,rooting and transplanting of tube seedlings,finally establish the clone of Viola verecunda.The results showed that MS＋BA0.4mg/L＋2,4-D2.5mg/L was the optimum medium for inducing callus from cotyledons and subculturing of induced callus.The optimum medium for callus and adventitious buds differentiation was MS＋NAAO.lmg/L＋BA0.6mg/L.1/4MS＋ABT 1/2 mg/L＋NAA0.3mg/L was the ideal medium for rooting and subculturing.The transplanting survival rate of tube seedlings was 91.9% and stable planting survival rate was 99.1%.Colonization of plantlets grew vigorously and maintained for all botanical traits of Viola verecunda.

中图分类号:

S567.23 Q943.1

杨丹刘姝孙靖靓宁淑香姜长阳. 堇菜无性系建立的研究[J]. 北方农业学报, 2012, (4): 33-33.