Abstract: In the study, the allelopathy of the secondary separation fraction of the aqueous extract of the root and the rhizosperic soil of parsley on Fusarium oxysporum.sp.cucumeris was studied by column chromatography and growth rate method. The results showed after the best one of the first separation fraction of the aqueous extract of the root of parsley was secondary separated and then the get fraction was respectively allelopathy test, the 9 promoted the growth of Fusarium oxysporum.sp.cucumeris; and the 1,2,3,4,5,6,7,8,10 inhibited the growth of Fusarium oxysporum.sp.cucumeris, and 1,5 showed the strongest inhibitory effect among them for the inhibitory rates of them were 37.83% and 28.64% and which were highly significant different with ck. After the aqueous extract of the rhizosperic soil of parsley in the same treatment with the aqueous extract of the root of parsley, all of the fraction inhibited the growth of Fusarium oxysporum.sp.cucumeris, and 1,5 showed the strongest inhibitory effect among them for the inhibitory rates of them were 30.83% and 38.04% and which were highly significant different with ck. The inhibition or promotion dropped down with time prolonged.