Abstract: Using buckwheat cotyledon node as the research object, its optimum conditions of regeneration was aimed to grope, to establish buckwheat regeneration system and lay the foundation for the research of transgenic buckwheat. The results showed that the best disinfection conditions was 0.1% HgCl2 for 1.Omin and 30g/L suerose＋7.5g/L agar medium on peeled buckwheat seeds, the best culture medium for cotyledon node induce buds was MS＋2.5mg/L6-BA＋ 0.05mg/LNAA＋30g/L sucrose＋7.5g/L agar and the best culture medium for buds to induce roots was 1/4MS＋2.0mg/L6-BA＋ 0.05mg/LNAA＋30g/L sucrose＋7.5g/L agar.